山羊血清中CD58含量的檢測(cè).pdf

1、西北農(nóng)林科技大學(xué)碩士學(xué)位論文山羊血清中CD58含量的檢測(cè)姓名：秦新喜申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：臨床獸醫(yī)學(xué)指導(dǎo)教師：靳亞平20080501DTECTIONTHECD58CONTENTINGOATSERUMABSTRACThl蚰ss硼y，thes1施ctantproteinCD58ofshe印redbl00dcells(SRBC)w嬲prep刪wittler億ymaticdigestionandcollmmcllrommo孕印llyRabbi

2、tSwereiIl加1uIliSedwiⅡlulemethodsofFreuIld’s刪uv觚t肌dl腫phoc歸adsorptionThetiterof觚ti—CD58semmw嬲tested謝tllthee渤blishInentindirectly鋤z)，Inelinkedimm玎oso舭nt嬲say(ELISA)1koptimisedwo幽ngconceIl仃ationsofcoated趾tigena11d鋤tibodywI珊pe

3、響姍ed謝nlpl謝觚xt脅tion1k砌№itionofindirectcornp成itiveELISAme吐10dsw弱establishedt0d烈ecttllelevelofCD58iIl90at∞mm，趾d瑚edt0detectthegoat∞mmleVelsofCD58es缸10哪cycleandanes臼粥咖odTheresultSwere弱follo、ⅣS：11kprep鋤tionofCD58、V弱te咖d塒ttlRos

4、etteInKbitionTe咄its砒曲itionEatew嬲tllepercentageof6201士206Ttle∞weretllI優(yōu)kindsofprotei璐innleCD58prepamtion五∞mSDSPAGE2ComparedtoFreuIld’sa由uV趾t，memetllodof1)，Inl)hocyteadsorptionw鶴obtainedspecificantibodies鋤ti—CD58，鋤tibodyle

5、velw嬲1：l103，t11emethodof髑ingceU如rfaCereceptor強(qiáng)dlig鋤dtoiIImmne鋤iII謝stoprepare鋤tibodyisfe嬲ible3111ei礎(chǔ)rectELISAmemod，coated鋤igenl0O嵋‘InL吐CD58diSsolVediIlc砷omtebuf】衙Solution(CBS，pH96，O05molL1)，塒tllHRP—labelledgoat鋤tirabbitIg

6、GdilutedbyPBSQH74，01mol‘Lq)iIll：2000弱a∞conda巧鋤tibody，1O％gelat訕】m嬲ablockingage盹w嬲developedt0detI獻(xiàn)antibodyagainstCD581kmethod’s畸)roducibili夠、v弱goodresunsanditscoemcientofV面abil時(shí)(Cv)w嬲248％～778％4Establi虹1menttl坨indireCtcompe

7、titiVeELISAtotesttheleVelofCD58iIlgoat骶：rum，meliI眥姍ge20肚5000000ng’IIlL～，regressioneq咖ionY=0207X1159(形=0988)，nle10westconce加眥ionofCD58is176ngmL一，wllichc鋤bedetectedⅡleoreticallyStatisticalar潮ysisindicatedtllattlle觚es曲吣aIld

8、也ees仃IlsperiodgoatsemmCD58contentissi嘶fic枷di彘rences(P005)Inaword，恤me廿lodofl腫phocytead涮ptiona11dindirectELISAcouldbeusedt0preparedSpecific鋤tibodiesanti—CD58鋤dtestt11econtI跚tofCD58iIlgoatse吣Theanes仃郴鋤dⅡleestr鄴p耐0d90ats鋤朋CD