PPARs激動劑羅格列酮對大鼠癲癇持續(xù)狀態(tài)海馬神經(jīng)元的保護作用.pdf

1、山西醫(yī)科大學碩士學位論文PPARs激動劑羅格列酮對大鼠癲癇持續(xù)狀態(tài)海馬神經(jīng)元的保護作用姓名：牛海雁申請學位級別：碩士專業(yè)：神經(jīng)病學指導教師：鄭輯英20100315碩士學位論文ProtectiVeEffectsofperoxisomeproIiferatorsactivatedreceptors(PPARs)agonistrosiglitazoneonhippocampaIneurotoninstatusepiIepticusratsAb

2、stractobjec廿Ve：PeroXisomeproli銜atorsactiVatedrec印torg徹a(PPARs)isaligaIld—activated仃alls謝ptionalfatorItplaySaneuroprotectiVer01eincerebralischenlicdisease，Parkinson’sdisease，舢zheimdsdisease，a11dothernefvoussyStemdiseasesb

3、yallti—inflaIIllllatoⅨaIltioxidatioll’a11ti—apoptotica11domermech粕ismswhenitisactivatedhlⅡlepresentinVeStigationweevaluatedtlleefrectsofPPARagonistrosi斟itazoncontoⅡlellippoc鋤p鄴ofstatus印i1印ticllsmtsBycon仃aStingtlleexpress

4、ionofBcl—2、BaXproteiIlalldⅡlenl枷berofn烈ELpositiVecellsinllippoc鋤叩lls硪erpiloCa印ine—inducedstanls印i1印ticus，t11eprotectiVee虢ctofrosi百itazone0nratsstatlls印ilepticIlScanbeevaluated觚dtllemechallismof廿1ismedicinecanbesnldiedMet

5、hods：Fifcy。follrhealtllyadultmaleWistarratswere姍domlydiVidedintotllree孕。oups：memodelControlgroup(11_24)，t11emodd仃e咖ent辱0up(n=24)(eachgroupisfhnherdiVidedintof0ursubgroupsd印eIldingonthetime，eachsubgroupsissix)aIldno肌alcon

6、協(xié)ol舯up(n=6)Eac_h罌’oupis仃eated、ⅣimmemethodofgavageThecontrol目oupandthemodelgroupare西venequalVolumesalineThemodel們她entgroupratsare百Vellrosi西itazonesolution(4mg／kg／d)Fivcday8mergavaged，Limi啪Chlodde(127mg俺，ip)waSⅫected18h塒or

7、tomeadmiIlis眥ionof舶鋤ypr印aredpiloc唧ine(30mg／kg，ip)1hecon咖l印upratsrecciVedall們a缸ll肌twitllsalineinsteadofLitlli啪C1lloride0rpiloc叩№111emodelneatIIlentgroupratSsllrViVedstatusepil印ticllsweregaVagedwitllrosi西itazo卿g／l(曲eve巧24h

8、ours，wrhilet11eratsofmodelCon仃olgroup鋤dnonnalconl∞l掣‘oupwere百vIeIlsalTleVolumeofsalineThemodelcon昀l孕ouparldthemodel仃ea缸n饑tgroupratsweresa翻ficed，respectiVely，at12h24h，72h，7daRerSETllecon仃0lgroup眺werekilledtogetherwimratst

9、llatweresa嘶ficed12hafterSEThehippoc鋤pusofallstudyobjectwere百VenTUNELstainingt0measureapoptoticcenand百Venimmunohistoch鋤ist拶鋤alysistotestBcl一2，Bax，attlles鋤etimeⅡleratioofBcl2／BaXw嬲calculatedSPSSl3Oversionwasusedforstatisti