國(guó)產(chǎn)熒光原位雜交(FISH)探針在產(chǎn)前染色體異常診斷中的應(yīng)用研究.pdf

1、貴陽(yáng)中醫(yī)學(xué)院碩士學(xué)位論文國(guó)產(chǎn)熒光原位雜交（FISH）探針在產(chǎn)前染色體異常診斷中的應(yīng)用研究姓名：朱珊珊申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：中西醫(yī)結(jié)合臨床婦科指導(dǎo)教師：黃元華20090601貴陌中醫(yī)學(xué)院碩士學(xué)位論文AbstractObjective：Toevaluatethefeasibilityofdomesticfluorescenceinsituhybridization(FISH)probesofchromosome13，21，18，XandY

2、inprenataldiagnosisofchromosomaldisease。Method：ProspectivestudyAmnioticfluidsamplesweretakenfiom85women缸l≯一32gestationalweekswhosefetuseswereathighriskofprenatalbirthdefects。Domesticprobesofchromosome13，21，18，XandYwereus

3、edtoexamineinterphaseamnioticfluidcells(AFCs)byFISH，withthecomparisonofkaryotypeanalysisbyGbandResults：85caseswereentirelynormalwithgoodhybridization，darkbackgroundandclearsignal，reportrateofkaryotypeanalysiswas9882％，dom

4、esticFISHprobeshadsignalexpressionrateof100％，thesignalquantityofvariousprobesandkaryotypeanalysisresultswereconsistent，andfalse—negativerateandfalsepositiverateof0In84successfulcasesofkaryotypeanalysis，thereWasonecaseofa

5、bnormalstructureon46，XX，4，一15，t(4；15)，mar，whichWaSnormalbyFISH。Conclusion：DomesticFISHprobesofctlromosomehadhigherefficiencyinthecytogeneticdetection，andcouldbecombinedwithcellcultureandkaryotypeanalysisforgeneticdisease