雙歧桿菌活的非可培養(yǎng)狀態(tài)的研究.pdf

1、吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文雙歧桿菌活的非可培養(yǎng)狀態(tài)的研究姓名：許婷申請學(xué)位級別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：錢愛東20080601StudyOnTheViableButNonculturableStateBifidobacteriumadolescentisAbstractTheBifidobacteriumadolescentisisprobiotics，mainlybeappliedinthenewgreenfeedadditive

2、whichappearedintherecentyearsIthasthecharacteristicofthenontoxicsideeffectnonresidual，nondrugresistance，nonpolluteenvironmentandSOonItsmainfunctionissupplementingmicroorganisminthequantityeitherthetypewhichtheintestinalt

3、ractreduced，toberestoremicrobiologybalanceoftheintestinaltractachievethegoalofhealthcare‘Theviablebutnonculturablestate”isakindofspecialsurvivalstatewhenthebacteriumunderthepoorsurroundingconditionsThebacteriumcouldnotbe

4、culturedbynormalmedium，butitisstillviableandhasmetabolicactivityUndertheadequatecondition，thebacteriumcouldrecovertheculturablestateThepurposeoftheexperimentisresearchingviablebutnon—culturablestateBifidobacteriumadolese

5、entis，takeitintotheresearchareaofprobiotics，findakindofmethodinproducingnewfeedadditivewhichpreventsillnessWedidresearchonthestateofviablebutnonculturabletotheBifidobacteriumadolescentisboughtfromChinaCenterofIndustrialC

6、ultureCollectionWithmanykindsofdetectionwaysFinallywegotthestablestatethatBifidobacteriumadolescentiscallbeinducedtoviablebutnon—culturablestateAndwedidpsychologybiochemistryand16sRNAtestandSOonWeprovedthebacteriumusedto

7、beinducedWaSBifidobacteriumadoleseentisBifidobacteriumadolescentisintheliquidLBculturemedium，pH=7，putsin4“CunderlowtemperatureanaerobicstaticcultivationthenentersViablebutnonculturablestateThe15clayslaterBifidobacteriuma

8、dolescentisentersViablebutnonculturablestate，ThestateCanmaintains嬲longas100daysTheLBculturemediumcarriesontheinductioncomparedwiththemediumofTPY，itCansavetheresources，reducesthecostandcompoundssimplyeasytooperate，easytop

9、reserveUsingtheexaminationofphysiologicalbiochemistrycharacterandtheappraisalisthemostearlymethodfordetectionandappreciationBifidobacteriumadolescentisitsalsothecrediblemethodatpresentTheresultofappreciationiscoincidence

10、thatthetargetwhichrespondedforallphysiologicalbiochemistrytalliescompletelywiththeBifidobacteriumadolescentistheorytargetItmaydeterminethisbacteriuminitiallyforBifidobacteriumadolescentisButbacterium’S16SrRNAconstantregi

11、onsequenceisbasicconservative，thereforeusingBifidobacteriumadolescentisconstantregionsequencetodesignprimerandtoamplificatethe16SrRNApartandmaythroughthissequencetoanalysis，itshomologytobe992％，thelastdeterminesthebacteri