水酶法提取玉米胚芽油和納濾技術(shù)回收蛋白質(zhì).pdf

1、江南大學(xué)碩士學(xué)位論文水酶法提取玉米胚芽油和納濾技術(shù)回收蛋白質(zhì)姓名：李新申請學(xué)位級別：碩士專業(yè)：食品科學(xué)指導(dǎo)教師：王璋20060601江南大學(xué)碩士學(xué)位論文AbstractAqueous廿lzymaticex訂actiontecllIlologyofcomge咖oilhaSbeenreportediIlmanyliteratIIres，however’itcouJdnotbe印pliediIlindllStryscalebecauseofi

2、tsdisadVantagesuchasaIa唱ev撕etyofem：ymesused，amassofen巧mesadded，alongtimereac缸o(hù)nperiodandlowoilyieldIntlledissenation，theoptim啪technoIogyandmechallismofaqueollse瑾螄aticextractionofcomge肌0ilhavebeenstlldied，onmebaseoftllis，

3、naIlofiltrationtechIlologyw硒aIsoappliedtorecoverandp嘣yproteiIl盤om也ehydrolysates，simllI切neously，simultaneously，meqllali哆ofproteinwasresearchedAboveall，t11etechnologyofaqueouseIlZymaticextractionofcomgeIIlloilwasdevel叩edWe

4、tcomge咖samplesweredispersedi11water(drysolid“waterratio1：6w～)Ci砸cacidwasaddeda11dhydrotlle肋a1treahncnt啪sdonebyautoclavingat110℃for1hThehydr礎(chǔ)盯mallytreatedcomgemlwasInilledbymeratio1：4(w／vdrysolids／water)togetsludgeofs鋤ple

5、s2O％(w／w)ofprotemaseand15％(w，w)cellulaSecn巧mesweresinlultaneouslyaddedtocommenceexⅡactionThehydrolysis、vascontifluedat40。Cfor2hfollowedbyanincreaseto50。C加dcontinuedfora110t11er8hAttheendofthee卿aticex勺哺ction，t11etcmpemtIl

6、reroseto80。CaIldheldfor15mintoinactiva艙t11eem了mes觚ercenmfIlgation，tlle矗eeoil，emtIlsion，hydml”atesandresiduesweres印aratedAttlleendofmcprocess，也eoilrecoverywaS937％of恤tinmeorigiIlalcomge肌111eSEMshowedtheprocessofhydrothenna

7、ltfe咖ema11den巧matice)(婦ctioncoulddrasticallydes在oytllesmJctllreofcomgenn，t11ent11eIipidcouldbereIeased丘nmthetissueoftllecomgemTheinnuenceoft11eaqueousen巧恤aticex仃actiontecllllologyofcomge咖oilontlleacidvaluewasstudied111ea

8、cid訓(xùn)ueoftlleorigiIlalcomgeHnoiI、懈50mgKOⅢg，afterbydrotllemal仃e釉em，mcacidvalueofmecomgcmoilroseto58mgKO№，butmeacidvalueofthecomgemloile)【tractedbytheaqueousenzymatictechnology、vasupto143mgK0ⅢgThehydrolyZationofthepmteinase

9、putalargeinfluenceonⅡ圯acidValueoftheoiLwIlilethehvdroth鋤al仃ea衄entallnosthadnoe丘缸o(hù)ntlleacidvalueoftlleoilnwasconcludedthattheacidicproteinaSeuSedine)【perimemcollldhydr01yzenotonlyp印tidelinkagebutalsoesterbond，WIlichcaused

10、丘℃efattyacidrelcaseandtheacidValuewasvcrylli曲ThecellulasereactioⅡc哪ed血eacidvaJueof也eoilch鋤geaIittleThepIof也eproteinhltheci伍cacidbu恐rwas55，aIldt】1ecimcacidbufferpHw觴about4，somecontentofsolubleproteiIliIl也ecitricacidbu飪erw

11、asveryllighSepharoseCL6Bgelfil缸齜ionofthesolubleproteiIlirIt11eemIllsionresultedmthJ就peakS，incorresponding誦mpeal【S，them01ecularweight(Mw)of也esolubleprote證focused0n305，562Da，120，254Daa11d75，440Da’duriIlgofwhich，t11epmteill

12、、^，imtheMwof12，0254Dawasmemaincomponent，andthepmportionofmepmtein(305，562Da)wasrelativelylowThe鋤lllsificationofmepmteinwasdifferemduetoitsMwTheNF2002540membraIlewasappIiedtorecovcftheproteinorpeptideinmecimcacidbllffhaIl