兒童急性白血病中PTENmRNA和hTERT、DNA-PKcs的表達(dá)及其意義.pdf

1、兒童急性白血病中P丁ENmRNA和hTERTDNAPK“的表達(dá)及其意義中文提要兒童急性白血病中PTENmRNA和hTERTDNAPKcs的表達(dá)及其意義摘要目的:研究抑癌基因、DNA修復(fù)基因及端粒酶在白血病細(xì)胞中的表達(dá)，探討其對(duì)白血病發(fā)生、發(fā)展的作用和臨床意義。方法:收集兒童急性白血病骨髓活檢標(biāo)本45例(包括初診組25例和緩解組20例)，以及對(duì)照組非白血病患兒骨髓活檢標(biāo)本20例。以骨髓組織石蠟切片作原位雜交PTENmRNA檢測，并用免疫組

2、化EliVision法檢測端粒酶催化亞單位(hTERT)和DNA依賴蛋白激酶催化亞單位(DNAPKcs)的表達(dá)。比較三組差異。結(jié)果:(1)PTENmRNA在初診組、緩解組和對(duì)照組陽性表達(dá)率分別為60.0%95.0%和100%，初診組失表達(dá)率顯著高于緩解組和對(duì)照組(P(3)DNAPKcs在初診組、緩解組和對(duì)照組陽性表達(dá)率分別為72.0%100%和100%初診組失表達(dá)率與緩解組和對(duì)照組差別顯著(P0.05).結(jié)論:兒童急性白血病顯示有PTE

3、NmRNA失表達(dá)、hTERT過表達(dá)及DNAPKcs失表達(dá)，表明抑癌基因、端粒酶和DNA修復(fù)基因在白血病發(fā)生中起到重要作用。本兒童急性白血病中PTEN.RNA和hTERT.DNAPKcs的表達(dá)及其意義英文提要StudyontheexpressionofPTENmRNAandhTERTDNAPKcsinchildhoodacuteleukemiaAbstractObjective:Studytheexpressionoftumorsuppr

4、essorgeneDNArepairgeneandtelomerasecomponentinacuteleukemiadiscusstheirefectsontheoccurrenceanddevelopmentofleukemiaandtheirclinicalmeaningsMethods:Collectmarrowbiopsysamplesof45childhoodacuteleukemia(including25firstdia

5、gnosedchildrenand20remissionchildren)，and20contrasts.ChecktheexpressionlevelsofPTENmRNAonparaffinembeddedsectionsofmarrowbiopsysamplesbyinsituhybridizationandchecktheexpressionsofhTERTandDNAPKcsbyimmunohistochemistry.Com

6、parethediferencesofthreegroups.Results:(1)PTENmRNApositiveratesingroupsoffirstdiagnosedremissionandcontrastare60.0%95.0%and100%lossexpressionrateoffirstdiagnosedgroupishigherthantheremissiongroupandcontrastgroupnotably(P

7、0.05P0.01)(2)hTERTpositiveratesingroupsoffirstdiagnosedremissionandcontrastare84.0%50.0%and40.0%positiverateoffirstdiagnosedgroupishigherthantheremissiongroupandcontrastgroup(P0.05P0.01)(3)DNAPKcspositiveratesingroupsoff