大豆降壓肽制備技術(shù)研究.pdf

1、河南農(nóng)業(yè)大學(xué)碩士學(xué)位論文大豆降壓肽制備技術(shù)研究姓名：蔡高峰申請學(xué)位級別：碩士專業(yè)：農(nóng)產(chǎn)品加工及貯藏工程指導(dǎo)教師：黃紀(jì)念；艾志錄201106河南農(nóng)業(yè)大學(xué)碩士論文StudyonPreparationofSoybeanAntihypertensivePeptidesSupervisor：ProfHuangJiNian，AiZhiLuMaster(PhD)Candidate：CaigaofengAbstract：Soybeanantihyper

2、tensivepeptidesarecompoundswithsmallmolecu1arweightobtainedfromenzymehydrolysisofsoybearlproteinStudyshowedthattheaminoacidcompositionofSoybeanantihypertensivepeptideswassimilartothatofsoybearlMeanwhileSoybeanantihyperte

3、nsivepeptidesWaShighlywatersolublerangewithlowviscosityandtheyweresuperiortosoybeanproteininbothnutritionalvalueandphysiologicalfunctionsAndtheeffectofloweringbloodpressureWaSsignificantTllisfeatureinthefoodandmedicinefi

4、eldshadwidelydevelopmentandresearchInthisexperiment，astherawmaterialofsoyproteinisolate，pretreatmentofrawmaterialsbythewayofwaterbathheating，andoptimizedbyorthogonaltestsSelecttheAlkalineproteaseandtrypsin，F(xiàn)lavourzymefor

5、hydrolysisofsoyproteinisolatetopreparesoyantihypertensivepeptidesStudyshowedthatantihypertensivepeptidesaregenerallypresentedintheformofsmallpeptidesandmorefrom2—12aminoacids，Inordertoobtainalargenumberofsmallpeptides，it

6、mustbefullyhydrolyzedmaterialinordertoincreasethedegreeofhydrolysisAsindextothedegreeofhydrolysis，Systematicstudiedthehydrolysisprocessconditions，focusingonthemainfactorsaffectthehydrolysistechnology，Suchasenzymedosage，t

7、emperature，pHandsubstrateconcentrationAtfirst，usedasingleproteaseforhydrolysisofsoyproteinisolate，usingresponsesurfacemethodtodeterminetheoptimalprocessparameters，Onthisbasis，studiedsynergybytwoenzymesandthreeenzymescomb

8、inedenzymatichydrolysis，preparationofoptimumconditionsofsoybeanantihypertensivepeptidesSoybeanantihypertensivepeptideswaspreparedunderoptimumconditions，thenpurificationofthemuseultrafiltrationandchromatographyanditsmolec

9、ularweightdistributionandaminoacidcompositionweredeterminedandresearchTheresultsareasfollows：1，Bystudyingtheimpactofpretreatmenttemperatureandtimeonthedegreeofhydrolysisandtheorthogonaltest，soyproteinisolateobtainedoptim

10、umtenditionsofpretreatment：pretreatmenttemperatureof70℃，pretreatmenttime10min2，Optimumconditionsofalkalineproteaseenzymolysisusingresponsesurface：Substrateconcentrationof342％，Enzymeconcentration600％，Hydrolysistemperature

11、46“CandpH981，Undertheseconditions，thedegreeofhydrolysisWas2715％3，Optimumconditionsoftrypsinenzymolysisusingresponsesurface：Substrateconcentrationof346％，Enzymeconcentration600％Hydrolysistemperature51℃andpH809Underthesecon

12、ditionsthedegreeofhydrolysiswas1248％4，Optimumconditionsofflavourzymeenzymolysisusingresponsesurface：Substrateconcentrationof316％，Enzymeconcentration562％。Hydrolysistemperature51℃andpH751Undertheseconditionsthedegreeofhydr