囊菌蟲草菌的分離、鑒定及其發(fā)酵工藝研究.pdf

1、浙江大學(xué)中清碩學(xué)位論文y776329囊菌蟲草菌的分離、鑒定及其發(fā)酵工藝研究碩士研究生：鄭靜導(dǎo)師：李永泉教授專業(yè)：生物化學(xué)與分子生物學(xué)⑧浙江大學(xué)生命科學(xué)學(xué)院文⑧AbstractCordycepsarekindsofimportantmedicinalfungusBecausetheecologicalenvironmentbecomesdeteriorated，thenaturalresourceofthesefungihasdeclin

2、edreapidlyinpopulationquantityandnowadaysmanyofthemhavedissappearedinmanylocationsinMainlandChinaArtificialcultureisarleffectivemethodthatintendstesolvetheshortageofnaturalresourceThenewstrainLi01ofCordycepswasisolatedfr

3、omthewildbodyfromXishuangbanna，Yunnanprovince，andthenitsfermentationtecheniquewasestabolishedTheactivitysubstancesoffermentationmycelinmweredetectedatthesametime1、ThestraincLi01WaSisolatedfromthewildfruitbodyofthefungusS

4、ubsequently，itWaSidemifiedbyitsITSsequenceandlocusinthedevelopmentprocesscombinedwitllmorphologycultureandphysiologicalcharacters2)FFDandRSAdesignswereappliedtopursuethemostsuitablemediumoffermentationTheoptimalmediumcon

5、sistedofsucrose66％，yeastextract1％，silkwormhydrolysate3％MgS047H20004％andKI[2P04O04％、Mththeoptimalmedium，theproductivityofCLi01wasraised267％Onthisbasis，theoptimalfermentationconditionWaSestablishedbyorthogonaltest，whichwas

6、temperature250C，inoculability13％，andprimarypH54；andthemyceliumofCLi01wasfurtherraised161％inshakeflasksafterfermentationconditionoptimization3)FermentationWasalsoscaledupina15Lfermemor，theresultshowedthatthebiomasshadnoob

7、viousdifferencebetweeninshakeflasksandinfermentorThekineticsmodelsofcellgrowthandsubstrateconsumptioninbatchfermentationprocesswereestablishedaccordingtoLogisticandLuedekingPiret—likemodel4)Thecontentofactivitysubstances