Basigin基因在小鼠子宮及卵巢中的表達與調(diào)節(jié).pdf

1、東北農(nóng)業(yè)大學碩士學位論文Basigin基因在小鼠子宮及卵巢中的表達與調(diào)節(jié)姓名：常紅申請學位級別：碩士專業(yè)：動物組織學與胚胎學指導教師：楊增明2001.5.1東北農(nóng)業(yè)大學ExpressionandRegulationofBasiginGeneinMouseUterusandOvaryABSTRACTBasiginbelongingtotheimmunoglobulinsuperfamilyhasbeenprovedtoplayanimpo

2、rtantroleinmousespermatogenesisandearlypregnancy.Inthisstudytheexpressionofbasigingeneinmouseuterusofearlypregnancywasfirstsystematicallyanalyzedbyimmunohistochemistry.Theexpressionandregulationofbasigingeneinmouseuterus

3、wasalsostudiedundertheconditionofpseudopregnancydelayedimplantationartificaldecidulizationandsteroidhormonetreatment.Inadditiontheexpressivepatternofbasigingenewasalsodetectedintheperiodoffollicledevelopmenttheformationa

4、ndregressionofCorpusLuteum(CL)bythemethodsofimmunohistochemistryandinsituhybridizationBasiginimmunostainingwasverystronginluminalepitheliumandglandularepitheliumofmouseuterusondayIofpregnancy.Thenbasiginimmunostainingwas

5、weakeninggradually.Itwashardtodetectthebasiginimmunostainingonday4ofpregnancy.Thestrongstainingwasseenintheembryosfromday5today8ofpregnancy.Onday5ofpregnancybasiginwasonlybeenseeninembryoesandluminalepitheliumandsublumin

6、alstromasurroundingtheblastocyst.Alowlevelofbasiginstainingwasdetectedinthesecondarydecidualizedcellsfromday6today8ofpregnancy.Duringtheestrouscyclethestrongeststainingwasseeninluminalandglandularepitheliumattheestrousst

7、age.Estrogensignificantlystimulatedtheexpressionofbasiginbothinluminalandglandularepithelium.TheexpressionofbasiginmRNAandproteinwasbecomingstrongerasthefolliclesdevelopedwiththestimulationofPMSGandhCG.Therewerenoexpress