2023年全國碩士研究生考試考研英語一試題真題(含答案詳解+作文范文)_第1頁
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1、安徽農(nóng)業(yè)大學(xué)碩士學(xué)位論文玉米和苜蓿全基因組WRKY基因的分析及進化研究姓名:江騰申請學(xué)位級別:碩士專業(yè):作物生物技術(shù)指導(dǎo)教師:朱蘇文201106IIAbstractWRKYproteinsareplantspecifictranionfactfamilynamedbyconservedstructuralregionofWRKYHighlyconservedaminoacidsequenceofWRKYGQK.ExpressionofW

2、RKYgenesarerapidlyinducedaftersufferinghurtpathogensabioticstressinmanyplants.TheresultsofsomeresearchshowedthatWRKYhadimptantroleinconductionofinnateimmunesignals.ThestudyonWRKYgenefromplantshasbecomeahotspot.Inthispape

3、rtheWRKYgenefromthewholegenomeofZeamaysMedicagotruncatulawasanalyzedtostudytherelationshipbetweengenestructureevolutionbybioinfmatics.Themainresultswereasbelow:1InthispapertakingmodelplantZeamaysastheobjectofstudybyHMMBL

4、AST126WRKYgeneswereobtainedinfinal.Thelatestwholegenomedatabase(Mt2.0)fromMedicagotruncatulawasstudied28WRKYgeneswerefound.2TheduplicationofWRKYgenesfromZeamaysMedicagotruncatulawasstudied.Theresultsshowedthattherewere84

5、duplicatedWRKYdomainsinZeamayswhichwas59.1%inthewholeWRKYdomains.Therewere19duplicatedWRKYgenesinMedicagotruncatulawhichwas57.3%inthewholeWRKYdomainslowerthanthatinZeamays.3BychromosomallocalizationofZeamaysthemostofWRKY

6、genesdistributionwasinchromosome2whichwas19.Thesecondwasinchromosome37whichrespectivlywere16.Therewere8WRKYgenesinchromosome5inMedicagotruncatulainwhichtherewere6genesinchromosome8whiletherewasnoWRKYgeneinchromosome26.4W

7、RKYdomainofZeamayswasanalyzedbyphylogeicsthedomainofNterminalCterminalofthefirsttypewasfoundinthesameevolutionarybranchwhichillustratedthattherewereindividualiginevolutionaryrelationshipsamongthem.Bythecomparisionofdomai

8、nsequencewecouldfoundthatthetypeⅠtypeⅡwasconservedwhichhadhighhomology.TheWRKYdomaininZeamaysSghumbicolwascomparedanalyzedtheresultsshowedthatNterminalCterminalofthefirsttypeclusteredtogatherwhichillustratedthedomainofWR

9、KYgeneofthefirsttypewasconservedmaybetheyhadcommonigin.WealsofoundthatthefirsttypewasthemostconservedWRKYdomain.5Therewere28WRKYgenesinthewholegenomeofMedicagotruncatulataking0.072%inallthegeneswhichwasmuchlessthanthatin

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