黑腹果蠅卷翅突變體分子機(jī)理的研究.pdf

1、Y940075分類號：嶄級：飛嚼差遺失雜‘博士學(xué)位淪義t々f℃碼：10u194彳q：B030iq2黑腹果蠅卷翅突變體分子機(jī)理的研究StudyonMolecularMcchanismofCurlyMutationinDrosophilaMelanogaster研究生：奎紅籃指導(dǎo)教師：連鹽生塾撞合作指導(dǎo)教師：昱堂信麴攫趙鲞塹副班盔雖申請學(xué)位門類級別：盛堂監(jiān)專業(yè)名稱：麴塑運(yùn)焦壹整皇鱉蕉研究方向：塾塹坌王堂量遮焦堂所在學(xué)院：塾塑型量堂瞳2006

2、年6月中國農(nóng)業(yè)大學(xué)博I：學(xué)位論文AbstractAbstractCurlymutationisdominantheterozygoteThegeneforCurlyisusuallylethalinhomozygousform，butCurlyisaeasilydistinguishabledominantmutantwingcharacterGiventhegeneforCurlyismultifunctionalondevelopm

3、entalprocessindrosophilamelanogaster，geneticistsmayelucidatemolecularmechanizeof“onegene，versatilefunction’’byinvestigatingwhatroleCygenecouldplayondifferentdevelopmentalperiodsindrosophilamelanogasterThe@mutationisthemo

4、stcommonlyuseddominantmarkerforthesecondchromosomebalancarsinDrosophilamelanogaster，butlittleisknownabouttheCygeneForconsiderationofthis，itisveryinterestingindrosophilageneticcommunitytostudyonmolecularcharacteroftheCyge

5、neInthepaper，Mendelian’slawofcurlymutationanditsmolecularmechanismwasinvestigatedsystemicallybymeansofgeneticcrossandmolecularmethodsGeneticCrOSSeSusedinthestudyincludecrossesoftwocurlystrainsandwildtypestrain，selfcrosso

6、fF1curlyfliesformcrossofthetwocurlystrainsandthewildtypestraincrossofthetwocurlystrains，theseresultsshowedthat：1)CurlyisadominantheterozygouscharacterthegeneforCurlyisusuallylethalinhomozygousform，meanwhilethehomologueof

7、itschromosomeintheCurlystockalsocontainsarecessivelethal，soundernormaltemperatureCurlymaintainsitselfinabalancedlethalstock；2)curlygeneisnotlinkedtopurplegeneinthecurlyflieswithpurpleeyes；3)twogeneofCurlyfromthecurlyflie

8、swithvermilioneyesandthecurlyflieswithpurpleeyesarealleleswhosetransheterozygoteislethalMolecularmethodsmadeinthepapercontainPC蹦polymerasechainreaction)，sequencing，RTPCR(reversetranscriptasePC心genepredictionandFQPCR(fluo

9、rescencequantitativepolymerasechainreaction)，whichallwereusedtodetectdifferenceatmeleeularlevelbetweencurlystrainsandwildtypestrainTheseresultsrevealedthat：1)A102一bpdeletionwhichislocatedbetweentheGenessynaptotagmin(svOa

10、ndAcfivinLikeProteinat23B口lp23功ontheDrosophilamelanogastergenomehadbeenfoundtobecommonlycontainedonCychromosomeinthreedifferentcurlymutantswhilea12bpinseaionlocatedatintronbetweenthethirdandthefourthexonswasfoundtoassoci

11、atedwithCychroraosot_rLeinthreedifferentcurlymutants2)itwasestablishedthatCygeneislethalinembryoperiodandanotherlethalonthecyhomologychromosomeismainlylethalinpupaperiod3)notranscriptionalgenecanbedeterminedonenessynapto

12、tagmin(syOandAetivinLikeProteinat23B凹lp23研ontheDrosophilamelanogastergenome，41expressionlevelforAIp23Bgeneinwildtypeisnothigherthanthatintwocurlystrainsonlarvalperiod，butexpressionlevelforAIp23Bgeneintwocurlystrainsishjg

13、herthanthatinwildtypestrainA11abovedmentionedshowedthatsomecommoncharacteratDNAlevelarecontainedonCvchromosome，itsuggestedthatdifferentcurlymutationscouldsharesomecommonoriginInterestingly，abnormalexpressionofAIp23Bgenec

14、ouldberesponsibleforcurlywing—isitpossiblethedeletionsomehoweffectsapromoter／enhancerintheActivinlikeprotein，resultinginabnormalexpressionorlackthereofinthewingdiscs，butthisneedmoreevidencetosuppoathehypothesisKeywords：D