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1、目錄摘要..........……,..............................................................……lAbstraCt......................……二................................................……!前言....................................................
2、.............................……11海洋天然產(chǎn)物的研究...........................................................................……22海洋微生物次級(jí)代謝產(chǎn)物的研究進(jìn)展.................................................……83海洋真菌次級(jí)代謝產(chǎn)物的研究進(jìn)展...............
3、....................................……103.1海洋真菌...........................................................................................................……103.2海洋真菌抗腫瘤活性代謝產(chǎn)物...........……,................................
4、.....................……n4紅樹植物內(nèi)生真菌及其次級(jí)代謝產(chǎn)物...............................................……144.1紅樹植物及紅樹植物內(nèi)生真菌.......................................................................……144.2紅樹植物內(nèi)生真菌次級(jí)代謝產(chǎn)物的研究.................
5、......................................……巧4.3紅樹內(nèi)生真菌A123及其次級(jí)代謝產(chǎn)物的研究186!.........................................……195高產(chǎn)菌種選育....................................................................................……205.1誘變育種....
6、.......................................................................................................……215.2育種工作高通量篩選方法...............................................................................……266培養(yǎng)基優(yōu)化..........
7、..............................................................................……287本課題研究目的、內(nèi)容和意義..........................................................……31材料與方法.........................................................
8、.............……321材料.................................................................................................……322方法................................................................................................
9、.……37結(jié)果與分析......................................................................……481菌株A123形態(tài)觀察............................................................................……481.1A123菌株的誘導(dǎo)產(chǎn)抱...............................
10、........................................................……481.2A123抱子OAPI染色觀察.................................................................................……481.3A123菌絲OAPI染色觀察.........................................
11、........................................……492原生質(zhì)體誘變條件優(yōu)化......................................................................……S02.1原生質(zhì)體制備......................................……,.........................................
12、.............……502.2原生質(zhì)體再生....................................................................................................……502.3原生質(zhì)體誘變...................……,.……,.,.....................................................
13、...........……503南強(qiáng)菌素高產(chǎn)突變株篩選方法的建立...............................................……SZ3.1依據(jù)表型差異的突變株篩選...........................................................................……523.2依據(jù)抗菌活性差異的突變株篩選..........................
14、..........................................……543.3依據(jù)TLc差異的突變株篩選............................................................................……553.4依據(jù)HpLC的突變株篩選...............................................................
15、...................……563.5質(zhì)譜(ESI一MS)................................................................................................……613.6抽NMR.............................................................................
16、..................................……613.7“抗菌活性一TLC一HPLC”聯(lián)用篩選方法確立...................................................……62I斤CatalogueAbst門CtinChinGSGAbstractinEnglish.........................……二...........................……
17、l!ntrdUCtiOn.........................................................................……1,Advanceonmarinenaturalproducte...........................................……22Advanceonsecondarymetabolitesfrommarinemicroorganism…8
18、3Advanceonsecondarymetabolitesfrommarinefungi............……103.1Marinefungi...............................……,..................................................……,o3.2Anti一tummetabolitesfrommanrinefungi...................
19、...............……114AdvaneeonmetaboIiteSfrommangroveendoPhyticfungi......……144.,MangroveandmangroveendophytiCfungi..................................……144.2SeeondarymetaboliteSfrommangroveendoPhytiefungi.........……154.3Se
20、COndarymetabolitesofendoPhytiefongusA123...................……195StrainSeleCtiOn.............................................................................……205.,Mutetionbreeding.........................................
21、.........................................……215.2HighThroughpUtScreening...................................................................……266Optimizati0n0fculturemediem..................................................
22、……287purpose,contentaandsigni打cance...........................................……3,MaterialSandmethodS..................................................……321MaterialS.......................................................
23、.................................……322MethOdS.........................................................................................……37Resulteandanalysis......................................................……481M0rph
24、0us0fstrainA123..............................................................……481.,Sporeinduced..................................................……,..............................……481.2Sporedyeing.......................
25、..................................................................……48,.3MyceliUmdyeing...................................................................................……492Optimizati0n0fpr0t0plastamutagenesis.........
26、.........................……502.1尸reparation............................................................................................……502.2Regenarati0n..................................................................
27、......................……502.3Mutagenesis..........................................................................................……503EstablishofHighThroughPutScreeningmethod.....................……523.,SCreeningdepentde
28、ntOnphenofypeofmutants...................................……523.2Screeningdepentdentonantimicrobial日ctMty.....................................……543.3ScreeningdepentdentonTLC................................................
29、...............……553.4Screeningdepentdenton日尸LC........................……,.............................……563.5ES!一MS..................................................................................................……613.
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