大豆貯藏蛋白亞基資源分析及年份地點(diǎn)變異研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文大豆貯藏蛋白亞基資源分析及年份地點(diǎn)變異研究姓名：姜振峰申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：作物遺傳育種指導(dǎo)教師：楊慶凱2003.5.1AbstractSoybeanisabundantinproteinbutdeficientinaminoacidscontainingsulfurwhichreducethenutritionvalueofsoybeanstorageproteinandrestricttlleabsorpt

2、ionanduseofsoybeanstorageproteininsidehumanbodyItispossiblethatvarietyofhi曲lISglobulinorhigll1S／7ScanbebredbyoneorlTiOrebreedingwayTheexperimentanalyzedt11edifierencesofsubunitstypeamongvarietiesandanalyzedt11econtentsof

3、subunitsofsoybeanstorageproteinbySDSPAoETheexperimentmaterialsconsistofbeth377varietiesoverseasandinlandand5varietiescultivatedl1locafionsincludingHeilon西iangprovince，JilinprovinceandLiaoningprovinceThemainresearchresult

4、sareasfollows：1ThebandtypesofGMaNbySDSPAGEaresameapproximatelyeachvarietyhave20bandsormoreThemainsubunitsconsistsofⅡ’、Ⅱ、n13、A3、Acid、basicandA5，themoleculeweiightofthesesubunitsis7745、6672、6044、4760、4071、3596、2088and1397K

5、DarespectivelyThe8subunitof7SglobulinofGMaxhasdoublebandtype；theacidandbasicsubunitshave6and4ormorebandsThisexperimentobtained1varietywhichcontaineddoublebandinpglobulinand3vailefteslackingonesubunitofacidandonesubunitof

6、basicandA5subunit2ThecontentsofdifierentsubunitsorthesarnesubunitofdifierentvarietiesvarygreatlyTheaveragecontentsofa’、a、丫、8andtotaJ7Sglobulinale658％、730％、466％、607％and2986％respectively，therangeofthosesubunitsare675％，946％

7、，785％，864％1915％respectively，andthevariablee幣cientofthosesubunitsare2091，2054，28742292，】288respectively；TheaveragecontentsofA3，A5，acidic，basicandtotalllSglobulinare46％，236％，28，49％，2197％，5826％respectivelytherangeofthosesub

8、unitsare657％，596％，1695％，2252％，291％respectively，andthevariableefficientofthosesubunitsare203，4278，1912，1525，994respectively3，Thevarianceof7Sglobulinoflocationsandvarietiesandyearsandtheinteractionbetweenlocationandvarieti

9、esandinteractionbetweenvarietyandyearjssignificantat005Ievelrespectively；Thevarianteof11Sglobulinoflocationsandvarietiesandyearsandinteractionbetweenvarietyandyearissignificantat005leve】respectively；Negativecorrelationsi

10、gnificantlyappearsbetween11Sglobulinand7Sglobulin；Themaxandminvalueof11S17Sofglobulinis370and134respectivelytheaverageis236ThevarianceofvarietiesandIocationsandyearsandtheInteractionbetweenyearandlocationof11S廠7Sissignif

11、icantotherinterantionsisnotsignificant；Thevariationamonglocatiousishi鐾hthanthatamongvarietiesin11S／7S，andthevariationofvarietiesof11S廣7SoverseasishigllthanthatinlandAIltheseshowthatvarietiesofhigh1lSglobulinorhigh11S／7Sc

12、anbebred4The11S／7Svalueof5locationsinHeilongiangProvinceincreasewiththeincreaseoflongitude5ThetypeofbandsofSDSPAGEimageamongGMaxandGsojaandbi空seedsoybean(229)andlittleseedsoybean(149)andgreensoybeanandbrownsoybeanissimil