硫酸銨飽和度的常用表,聚丙烯酰胺凝膠的配制表

1、硫酸銨飽和度的常用表硫酸銨飽和度的常用表1調整硫酸銨溶液飽和度計算表（0℃）在0℃硫酸銨終濃度，%飽和度20253035404550556065707580859095100每100ml溶液加固體硫酸銨的克數(shù)010.613.416.419.422.625.829.132.636.139.843.647.651.655.960.365.069.757.910.813.716.619.722.926.229.633.136.840.544.

2、448.452.657.061.566.2105.38.110.913.916.920.023.326.630.133.737.441.245.249.353.658.162.7152.65.48.211.114.117.220.423.727.130.634.338.142.046.050.354.759.22002.75.58.311.314.317.520.724.127.631.234.938.742.746.951.255.7

3、2502.75.68.411.514.617.921.124.528.031.735.539.543.647.852.23002.85.68.611.714.818.121.424.928.532.336.240.244.548.83502.85.78.711.815.118.421.825.429.132.936.941.045.34002.95.88.912.015.318.722.225.829.633.537.641.84502

4、.95.99.012.315.619.022.626.330.234.238.35003.06.09.212.515.919.423.026.830.834.85503.06.19.312.716.119.723.527.331.36003.16.29.512.916.420.123.127.96503.16.39.713.216.820.524.47003.26.59.913.417.120.9750326.610.113.717.4

5、8003.36.710.313.98503.46.810.59003.47.09503.5硫酸銨初濃度，％飽和度1000表1配制Tris甘氨酸SDSPAGE聚丙烯酰胺凝膠電泳分離膠所用溶液不同體積（ml）凝膠液中各成分所需體積（ml）溶液成分5101520253040506%水2.65.37.910.613.215.921.226.530%丙烯酰胺溶液1234568101.5molLTris(pH8.8)1.32.53.856.37.5

6、1012.510%SDS0.050.10.150.20.250.30.40.510%過硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.048%水2.34.66.99.311.513.918.523.230%丙烯酰胺溶液1.32.745.36.7810.713.31.5molLTris(pH8.8)1.32.53.856.37.51012.510

7、%SDS0.050.10.150.20.250.30.40.510%過硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0030.0060.0090.0120.0150.0180.0240.0310%水1.945.97.99.911.915.919.830%丙烯酰胺溶液1.73.356.78.31013.316.71.5molLTris(pH8.8)1.32.53.856.37.51012.510%SDS0.05

8、0.10.150.20.250.30.40.510%過硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.0212%水1.63.34.96.68.29.913.216.530%丙烯酰胺溶液2468101216201.5molLTris(pH8.8)1.32.53.856.37.51012.510%SDS0.050.10.150.20.250.30.