馬立克氏病病毒UL13蛋白激酶的體外表達及抗體制備.pdf

1、揚州大學碩士學位論文馬立克氏病病毒UL13蛋白激酶的體外表達及抗體制備姓名：蘇鈺文申請學位級別：碩士專業(yè)：預防獸醫(yī)學指導教師：劉岳龍秦愛建20090501揚州大學碩士學位論文InVitroexpressionandantibodypreparationofMarek’sII—diseaseVims(MDV)UL13proteinkinaseMSCarldidate：ⅥJwenSuSupeⅣisor：Y，uelongLiuAijianQi

2、nAbstractBaCtoBac@BaculovimsExpressingSystemis蜀汜quemly印pliedt0generateheterogonousproteiIlSo、vingt0itsspecialcharactersofhi曲leVelsofheterogonousgeneexpressionandhighbioaCtiVi吼aIldmepllIifiedproteinarecontributedtot11e觚al

3、ysisofproteinbio如nctionThebiofhnctionofconservedhe印esViralproteinl(inaSes(CHPKs)inherpesVimsareoneoftllehottopicofhe印esVims’researchT11erolesofdi毹remCHPKsplaydifrerently鋤ongdifrerenthe印esViridaeInthis殉Jdy，BaCtoBac@Baculo

4、viruSExpressingSystemw舔印pIiedtoexpressthe朋口旭七≥如P儺P1，驢姍(MDV)UL13proteinl(inasewhichisamemberofCHPKsThreerecombinantbaculovimsesincludingrBacHis—ULl3，rBac—HisEGFPandrBacHisWereconstructedSuccess如llyOpt婦alexpressionconditio

5、nsandharVesttimeforMDVULl3prmeinl(inauseauldEGFP、Vereconfi肌edbyoptimizationassaysInthisstud弘也e叫ficationplatfomofBactoBac@BaculovimsExpressingSystemwaSpreliminarilyestablishedbasedonBaCtoBac@HTvectorsBesides，theessentialm

6、atedalsforMDVULl3proteinkinaSebio—functionresearchwereobtainedToobserVet11een’ectSontheViral黟owthofMDVaLssays、ⅣereperfoHIledthrou曲MDVUL13proteinkinaSeuptal【eTheresultsshowedthatMDVUL13proteinkinaSehadnosi鰣ficante髓ctsontl

7、leplaquesizeofaVeD，Vimlent(w)MDvlstrain—RB一1BPolyclonalamibodyagainsttlle血UUL13ORFwaSpreparedbygeneilIl】mImizationAntibodiesagainstMDVULl3proteinkinaSegeneratedviadifrerentmethods、Ⅳere印pliedtodetectedMDVULl3proteinkinaSe