水稻生物信息數據庫及本地化生物信息分析平臺的構建.pdf

1、華中農業(yè)大學碩士學位論文水稻生物信息數據庫及本地化生物信息分析平臺的構建姓名：張利達申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：張啟發(fā)2002.5.1AbstractⅥ’euselarge—scaleESTssequencingtostudylevelsofgeneexpressionassociatedwithcultivatedtraitsToperformlarge—scaleanalysisofthelargeamou

2、ntsofthesequencesanddiscovernewgenes，weconstructedanintegratedbioinformaticanalysissystem1ConstructionofanintegratedESTsanalysissystembasedonlocalBasedonLinuxoperatingsystem，thePhred／Phrap／ConsedsoftwareandBLASTsoftwarew

3、ereusedtoconstructaplatformforbatchanalysisofthesequences，includingBasecalling，contaminateremoving，repeatsequencesmasking，sequenceassemblyandsequencesimilarityanalysisMoreovergeneidentificat!on，transcriptioncontrolregion

4、analysis，multiplesequencealignment，phylogeneticanalysis，simplesequencerepeatidentificationandprimersdesigningarealsoincludedOnthebasisofthese，WebinterfaceofBLASTsoftwarewasconstructedforvisualizedsequencesimilarityanalys

5、is，alloftheusersintheintranetcouldvisittheWebBLASTserverusingbrowsertoanalysistheirsequenceeasily2StudyinESTclusteringmethodandapplicationinriceWedevelopedanESTclusteringmethod，ESTClustering，togeneratehigh—qualityuniquee

6、xpressedsequencebasedonlarge—scaleESTsequencingThemethoduseconsensussequencestosequenceanalyzewithmegablastandassembleeachclusterwithphrapinclusteringprocessTheclusteringstrategycanefficientlyidentifygonefamilyandalterna

7、tesplicingformsofexplesscdseqtlcncesItcanalsoreducetheadverseeffectscausedbysequenceerrors’lheFS1Clustm’ingmethodtendstoprovidemoreexpressedgoneformscomparingwiththe【lniGcneclusteringmethodoftheNationalCenterlbrBiotechno

8、logyInformationAnalysisofthe112256ESTsofA／‘abidopsiswithESTClusteringproduced23，581ESIclustersAmongtheseArabidopsisESTclusters，13597havecorrespondinggenomecodingsequencesandthisnumberisclosetothenumberofgenespredictedwit