生物素缺乏致肉雛雞肝腎綜合征發(fā)病機理的研究.pdf

1、河南農(nóng)業(yè)大學(xué)碩士學(xué)位論文生物素缺乏致肉雛雞肝腎綜合征發(fā)病機理的研究姓名：趙帥兵申請學(xué)位級別：碩士專業(yè)：臨床獸醫(yī)學(xué)指導(dǎo)教師：仝宗喜20100601河南農(nóng)業(yè)大學(xué)2010屆臨床獸醫(yī)學(xué)碩上學(xué)位論文STUDYONTHEMECHANISMOFLⅣERANDKIDNEYSYNDROMEINCHICKENBYBIOTINDEFICIENCYSupervisor：TongZongxiMasterCandidate：ZhaoShuaibingABSTRAC

2、TBiotinisakindofvitaminecontainingwithSulfurthatisnecessaryforanimalhealth，andlackofbiotinwouldresultinslowgrowthofanimals，ingestationdecreasingreductioninfemaleproduction，decreasingincarcassquality，dermatitis，andevendea

3、thofanimals，whichcausegreateconomiclossinlivestockandpoultryraisingindustryBiotindeficiencywasacommondiseaseofbroilerchickens，layers，pigs，andetcChickenslackofbiotinwillleadtoinflammationofmouth，bloodingininterdigit，short

4、andthickinshinbone，HepatorenalSyndromeandSOonThepurposeofthisexperimentWaStostudythepathogenesisofHepatorenalSyndromeinchickenscausedbybiotindeficiencyAtotalof50doubleAchickenswererandomlydividedinto2groups，theexperiment

5、algroup(biotindeficiencygroup)andcontrolgroup：Grouplwastheexperimentalgroupwith30chickensfedwiththedietofbiotindeficiency(preparationofsemipurifieddiet)；Group2Wasthecontrolgroupof20chickensfeedingthedietsupplementedwithb

6、iotinof500pg／kgAndthechickensofexperimentalgroupandcontrolgroupofod，5d，15dwereweighed，thedeadchickenswereslaughteredandthedetailedpathologicalchangesWaSrecordedbycameraFivechickenswereexaminedintheexperimentalgroupandcon

7、trolgroup，respectivelyandthebloodroutine，liverfunctionandkidneyfunctionweretested，thentheexperimentalchickenswerekilled，andtheliver，spleen，kidneyandheartofthechickensweretakenout，weighed，andtheorgancoefficientofchickensw

8、ascalculatedAndaportionofliverandkidneyweresampledforpathologicalexaminationandtheexpressionofholocarboxylasesynthetaseWastestedTheresultsshowedthat：(1)Theclinicalsymptomsofbiotindeficiencyinchickenswereconsistentwiththe

9、reportedresults(2)Biotindeficiencyseverelyinhibitsthegrowthrateofchickens，thedifferenceisverysignificant妒D00；(3)ThedevelopmentofchickenspleenWasaffectedbybiotindeficiencyandthedifferenceissignificant(PDOD；ThefatWasdeposi

10、tedintheliverkidneyandheart，whichleadtoincreaseoftheorgancoefficientofchickens，thedifferenceofkidneyandheartisverysignificant妒DDJ)，thedifferenceofliverissignificantPDI”_；(4)Theliverfunctionisimpaired，andalbuminandtotalpr