巖藻聚糖硫酸酯酶產生菌篩選和酶學性質的研究以及巖藻寡糖的應用研究.pdf

1、中國海洋大學碩士學位論文巖藻聚糖硫酸酯酶產生菌篩選和酶學性質的研究以及巖藻寡糖的應用研究姓名：霍立華申請學位級別：碩士專業(yè)：水產品加工與貯藏指導教師：薛長湖2003.6.1ScreeningoffucoidanaseproducingbacteriaandstudyonitsenzymecharactersandtheapplicationofoligosaccharidesAbstractFucoidansis硅uniqueclass

2、ofsulfatedpolysaeehalide娟丞manyspecialactivities。Andoligosacchaddesfromfucoidansarcmorevaluablethanthewholefueoidarmduetotheirlowconglufinationand確務solubilityInthispapersamplesalecollectedfromdifferentcoastalareasinQingda

3、oinordertoobtainbacteriaproducingfucoidanaseAfterenriching，separatingfirstscreeningandsecondscreening，lndueedmutationandscreeningafterinducedmutation，fivestrainscapableofproducinghigllfucoidanaseacGvities勰separatedandsel

4、ectedAmongthem。thes娃ainAhasthehighestactivityandthefucoidanaseactivityisashighas368UAfterthethalliandcolonyfeaturesaleanalyzedthesl：rainAisidentifiedtobeEosipetothrixRosenbachaccording幻Bergey’SManualofDeterminativeBacter

5、iologyBesides，theoptimalculturemedia，theoptimalfermentationconditionandsomefactorseffectsOnfucoidanaseactivityareinvestigatedthroughthesinglefactor船stBystudyingcharacteristicsoffucoidanase，aconclusionhasbeenmadethatthefu

6、coidanasehasahi酶thermalstabilityand拉stabilityAndfucoidanase鋤retainitsactivityUSder50centigradeandwithinpH47Atthesa艇letimethestudyshowsthat赫礦Canpromotethefucoidanaseactivitygreatlywhenitsconcentrationisuptol10一mol／1Inaddi

7、tion，theformationofcerium(IV)otigosaccharidecomplexesandeatal辯ichydrolysis&collagenbythecomplexesarealsodiscussedinthisresearchAfterfucoidansdegradingoligosaccharideswithdifferentmolecularwei#tF|，F(xiàn)2andF3，alegained。Themol

8、ecularweightrangeofFl，F(xiàn)2andF3alemorethan5000，from1000to5000andtessthant000respectivelyTheresultshowsthatF3isobviouslyeasytoformthecomplexwithceriumcomparedwithF2andF3Andtheeerium(IV)F3complexhasmuchgreatercatalyticactivi

9、tyofhydrolysiscollagenthanceriumOV)一Ftandcerium(IV)F2complexes，MoreovertheoptimumratioandtheoptimumhydrolysisconditionsarealsoobtainedinthisarticleKeyword：Fueoidan；Fueoidanase；strain；cerium(IV)oligosaccharldeeomplex；coll