中國(guó)對(duì)蝦血細(xì)胞單克隆抗體的研制及其應(yīng)用.pdf

1、青島海洋大學(xué)碩士學(xué)位論文中國(guó)對(duì)蝦血細(xì)胞單克隆抗體的研制及其應(yīng)用姓名：張利峰申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：水產(chǎn)養(yǎng)殖指導(dǎo)教師：戰(zhàn)文斌周麗2001.6.1青島海洋犬掌水產(chǎn)掌院碩‘論文2001——————●——————_——_———_——————_——、●———H——●—_———_^—_—●———_——_——●—————————●—●—●——————————————————一一AbstractInordertostudyimmunecellulare

2、ffectorsinshrimp(Penaeuschinensis)，threetypesMcAbswereproductedbyimmunisingBalb／cmousewithhaemocytesthatcarlidentifythreehaemocytesubpopulationsofPenaeuschinensis，andusingthoserevealedtheimmunecharacterizationofreceptorl

3、ocatedonthethreehaemocytesubpopulationsofPenaeuschinensis，themainmeansasfellow：Inthisstudy，Balb／cmicewereimmunizedwiththehaemocytesofshrimp，polyethyleneglycol(PEG)wasusedassolutionforcellfusion，andhybridomaswasselectedby

4、meansofenzyme—linkedimmunosorbentassays(ELISA)andImmunofluorescenceassays(IrA)Twenty—threestrainsofhybridomaswhichproducemonoclonalantibodiesagainsthaemocytesofshrimpTherearethirteenstrainhi【曲一affinityMcAbswereselectedby

5、IFA，andusetheMcAbscanidentifythreetypeshaemocytesofshrimp，3A3、3A2、3A5、3A6、382and585McAbsshowedspecificallyreactivitywithhyalinecellandrecognisedthereceptorlocatedonthesurfaceofhyalinecelli383、3C2、3C4、5A2and582McAbsspecif

6、icallyboundtosmallgranularcellwhilerecognisingthecellmembrancereceptorlocatedonthesurfaceandcytoplasmreceptorofsmallgranularcell386、4A4McAbsreactedonlywithlargegranularcellandwereabletecognisethecellmembranereceptorwhich

7、locatedonthesurfaceoflargegranularcellInthisstudyusingtheproducedMcAbstevealthegenesisanddistributionofhaemocyteinpostlarve(Penaeuschinensis)，theresultsisthattherearemanyofhyalinecellsintheantenna，thegatheringcellsindica