廣東魴與團(tuán)頭魴的雜交育種研究.pdf

1、上海水產(chǎn)大學(xué)碩士學(xué)位論文廣東魴與團(tuán)頭魴的雜交育種研究姓名：葉星申請學(xué)位級別：碩士專業(yè)：水產(chǎn)養(yǎng)殖指導(dǎo)教師：樓允東謝剛2000.6.1AbstractBreedsarethematerialbasesofaquacultureandselectionandbreedingofgoodbreedsareaneffectivewaytopromoteproductionTraditionalbreedingincludeshybridizati

2、onandselectivebreeding，Inthisresearch，distanthybridizationisadoptedMaleMegalobramahoffmanniandfemaleMegalobramaamblycephala，whichbelongtoCyprmidae，Megalobramaarechosenasparentsinordertogetbreedhybridwithheterosisnmresear

3、chworksincludethefollowingaspects：1)Crossfertilization；2)Morphologicalcomparison；3)Growthcomparisonexperiment；4)Analysisandcomparisonofkaryotype；51ComparativestudyonsixisozymespattersinthreetissuesTheresultsareasfollowsl

4、TheCrossfertilization：ThehybridwassuccessfullybredthroughCROSSfertilizationMegalobramahoffmanni(9)XMegalobramaarab蜘ephala(6)1kfertilizationratewas75％；thehatchingrateWas67％：andabnormallarvaaccounted29％Theprocessofembryomc

5、developmentlaSted3037hoursat25270cThehybrid(FI)isfertileandmaturesintwoyearsmGuangdongareaundercultivation2Themorphologicalcomparison：ThehybridismorphologicallysimilartoitsparentsItsboayshapeislikethatof膨haffraanniAndits

6、eyediameterislargerthanthatof膨hoffmannibutsmallerthanthatofMamblycephalaItsbodycolourismediatedbetweenthatoftheparentsMostofcountableandmeasurableparametersofthehybridaresimilartothoseoftheparents3Thegrowthcomparison：The

7、growthrateofthehybridlarvaisbetweenthatofitsparents111eheterosisisnotsignificantTheordersofbodyweightinstantgrowthrate(IGR。)，bodylengthinstantgrowthrate(1G氏)andgrowthindex(I)fortheparentsandthehybridaregamblycephalathehy

8、brid膨hoffraanni4Analysisofkaryotype：Thekaryotypeofthehybridissametothatof膨hoffmannithatis26m18sm4stThecharacteristicsofkaryotypesoftheparentsandthehybridaremainlymetaeentricandsubmetaeentriechromosomesTherelativelengthof

9、chromosomesfortheparentsandthehybridisverycLoseAndthebiggestpairsofchromosomesarethenumberl，whicharesubmetacentricBythekaryotypeofthehybrid，itisdifficulttodistingutshtheoriginationofthehybridchromosomes5Analysisofisozyme

10、s：Sixkindsofisozymesinthreetissues，namelyESTLDH，MDH，M3H，SODandIDH，i【ltheparentsapparentlyexhibitatissuespecificandspeciesspecificexpressionAmongthem，ESTMDHADHandIDHat@themostactivein／i1hoffm鯽／ziMostoftheisozymesexpressed