30061.草魚激活素ⅰ型和ⅱ型受體的cdna克隆、結構分析及其在垂體細胞中的表達調(diào)控的研究

1、電子科技大學碩士學位論文草魚激活素Ⅰ型和Ⅱ型受體的cDNA克隆、結構分析及其在垂體細胞中的表達調(diào)控的研究姓名：宋春雷申請學位級別：碩士專業(yè)：生物物理學指導教師：周紅20090501ABSTRACTABSTRACTSince廿leinitialdiscoveryactivincumulativeevidencefurtherhasestablishedthatactivinanditstwofunctionalmodulators，the

2、activinbindingproteinfollistatinandtheactivinantagonistinhibinregulatedreproductivefunctionbyexertingeffectsatalllevelsofthereproductiveaxis，inparticularattheovaryandpituitaryHowevertheintracellularmechanismsforactivinac

3、tions，egreceptorexpressionlevels，havenotbeenexaminedInthepresentstudy，twocDNAsencodingtheactivintypeliBreceptor(ActRIIB)andactivintypeIBreceptor(ActRm)wereclonedandcharacterizedfromgrasscarpThededucedActRIIBproteinof510a

4、minoacidsshared7990％identitywiththoseinothervertebrates，whilethepredictedActRIBproteinof505aminoacidsexhibitedhighsequenceidentity(8096％)toitscounterpartsinhuman，rat，mouse，flogandzebrafishPhylogeneticanalysisrevealedthat

5、tworeceptorswerehighlyconservedduringvertebrateevolutionFurthermorecomparativeanalysisshowedthateachofthesetweceptorscontainedtheconservedaminoacidresiduesrequiredforactivinbinding，andcomprisedthecharacteristicregionsofa

6、nextracellularligandbindingdomainasingletransmembraneregionandanintracellularserine／threoninekinasedomainThehighdegreeofconservationofreceptorfunctionaldomainsbetweenhumanandgrasscarpsupportstheideathathumanactivinAusedi

7、nthepresentstudycouldinteractwithgrasscarpActRIIBandActRIBRealtimePCRanalysisrevealedthatbothActRIIBandACtRIBtranscriptswereubiquitouslyexpressedinallofthetissuesexamined，includingpituitaryUsingastaticincubationapproacht

8、hedirectactionsofexogenousactivinonitsownreceptors，ActRIIBandActRIB，mRNAexpressionwereexaminedatthepituitarylevelActivinsignificantlystimulatedmRNAexpressionofgrasscarpActRIBinatimeanddosedependentmanner，butdidn’talterth

9、eexpressionlevelofActRIIBThesefindingssupportthenotionthatactivinreceptorsmayserveasalocalregulatorypointinvolvingpituitaryfunctionofactiviningrasscarpInadditionexpressionplasmidsforkinasedeficientformandconstitutivelyac