甘藍型油菜矮稈突變體及矮稈基因分子標記的研究.pdf

1、華中農(nóng)業(yè)大學碩士學位論文甘藍型油菜矮稈突變體及矮稈基因分子標記的研究姓名：黃天帶申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：吳江生20030601華中農(nóng)業(yè)大學2003屆碩士論文StudiesonaDwarfMutantandMolecularMarkersofDwarfGeneinBrassicanapus三AbstractThemaingermplasmusedinthisstudywasadwarfmutantnamedDs一1w

2、ithzhongshuangNO4(ZD4)andHuashuangNO3(HD3)ascontr01CharactersoftheyoungseedlingofDs1wereinvestigatedunderlightanddarkconditions，anditsothergrowinghabitsandagronomiccharacterswereidentifiedinfieldThereactionsofitshypocoty

3、landstemtodifferentphytohormonetreatmentswereobservedTheinheritanceofthegenefortheshortstemofDs1andtheallelicrelationshipwithanotherdwarfgermplasmDs2werestudiedRAPDtechniqueandcandidategeneapproachwereadaptedtodetectmole

4、cularmarkerslinkedtothedwarfgene(Dsl)Themainresultswereasfollows：1HypocotyllengthofDs一1grownunderlightconditionwasshorterthanthatofZD4，butnoobviousdifferenceunderdark；Ds1waslowersignificantlythanZD4inplantheightbranching

5、position，numberofelongatednodes，lengthofintemodeandmaininflorescence；butastothenumberoftotalnodesthereWasnosignificantdifferencebetweenthedwarfandthenormalvarietiesItshowedthatthedwarfphenotypewasMuetofewernumbersofelong

6、atednodes，shorterlengthofintemodeandmaininflorescence，lowerbranchingpositionsIncomparison謝tllthecontr01DsIhadfewersiliquesperplant，loweryieldperplantandhiger1000seedsweight，butnosignificantdifferenceexistedinbranchnumber

7、s，podsofmaininfloresenceandseedsperpod2Phytohormone6BA、BRhadnosignificanteffectstothehypocotyllengthofDs一1：L蛆andGA3enhancedhypocotylelongationsignificantlybutonly100mg／LGA3couldelongateitshypocotyltonormallength；6BA、IAA、

8、NAA、GA3、920、BRhadnoobviouseffectstothestemelongationofDs1，while600megLPP333reducedthestemlength，and600mg／LGA3couldpartiallyenhancethestemlengthreducedbyPP333butnottonormalTheseresultsindicatedthatthesignaltransductionfor

9、GA3inDs1wasnormal3GeneticanalysisshowedthattheplantheightsofF1populationwasintermediateoftwoparents，butpartialtodwarfparent，F(xiàn)1andRFlhadnosignificantdifferenceThesegregationratiooftallplants：shortplantsinF2populationaccor