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1、四川農(nóng)業(yè)大學碩士學位論文水稻葉片黃化矮稈突變體的遺傳分析與基因定位姓名:王玉平申請學位級別:碩士專業(yè):作物遺傳育種指導教師:李仕貴黎漢云20030501Geneticanalysisandgenemappingofadwarfmutantwithyellowleafinrice(OryzasativaL)ABSTRACTRiceheightcharactersareoneofthemostimportanttraitsofriceIti
2、snecessaryforricegeneticsandbreedingtodiscoverandresearchnewdwarfgeneAdwarfmutantwithyellowleafwasfoundedinricebreedingfarmWithseveralsemidwarfvarietyandatallvarietysomeresearchaboutthedwarfmutantwerecarriedoutThemainres
3、ultsaresummarizedasbelow:1Themutantintheseedingandtheshootingstagewereseparatelytreatedwithgibberellinacid(GA3),itWaSsensitivetoGA3treatmentintwostageTheheightofthemutantincreaSedanddidnotrecovertothenormalheightTreatedw
4、ithGA廿,theseedsettingrateofthemutantWaSimproved2InthisstudyGeneticanalysisforthemutantinFlandF2populationrevealedthatthedwarfismiscontrolledbytworecessivegenesAdwarfgenewassd1,theotherwasnotItwasconcludedthatthemutant’Sg
5、enotypeWaSsdlsdldw∞正w似andthereWaSepitatiecessivenesstothegeneofSd1andsd13Withthenearisogeniclines(NILs)ofthemutant,theeffeetionofthedwarfmutantwereresearchedthereductionofplantheightinthepresenceofdwarfinggeneismainlyexp
6、ressedthroughtheshorteningonthelengthsofthreeupperintemodesTheshorteningofpanicleandtheincreaseoftillerareprobablyduetothepleiotropyofthedwarfinggene4BulkedsegregantmethodandCO—segregationanalysisinF2populationwereusedto
7、screenmolecularmarkerslinkedwiththedwarfgeneItwasshowedthatthedwarfgenelayinchromosome9TheresultshowedthatthemarkerRM3l6inchromosomewaslinkedwitllthetargetgeneandthegeneticdistanceis8,lcMThusthisresearchisthefirsttomapan
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