鰱早期性分化與性轉(zhuǎn)及兩個雌核發(fā)育系近交F-,1-代遺傳多樣性的研究.pdf

1、華中農(nóng)業(yè)大學碩士學位論文鰱早期性分化與性轉(zhuǎn)及兩個雌核發(fā)育系近交F代遺傳多樣性的研究姓名：張桂蓉申請學位級別：碩士專業(yè)：水產(chǎn)養(yǎng)殖指導教師：嚴安生鄒桂偉20040501AbstractSilvercarp(Hypophthalmichthysmolitrix)isoneofthemainfarmedfreshwaterfishandpossessalargeproportioninfreshwateraquacultureinChinadu

2、etotheadvantagesofitsshortfoodchain，easilyrearingandlowCOStdumgfamlillgAtthepresenttimebecauseofthelackoftechniquesinseedselectionandseedprotectionthenaC刪rCSOUrCCSofsilvercarp、vcredestroyedandthepropagationsincloserelati

3、vesleadtothegermplasmdegenerationResearchersintheYangtzeRiverFisheriesResearchInstituteapplythemselvestotheseedselectionandbreedingofgynogeneticsilvercarpallthetimes，andhavepreliminarilyestablishedtwoartificial8ynogeneti

4、cstrainsatpresentbasedonmolecularmarkertechniquesButtheSUCCESSininducinggynogenesisisonlythefirststeptoachievebreedingselectionofgoodstraininsilvercarp，Wemuststillsolvetheproblemsinreproducingoffspringandkeepinggoodchara

5、ctersofparentsDuringtheperiodfromMaytoDecemberin2002，histologicalmethodw船usedtostudytheearlygonadaldifferentiationofsilvercarp，illordertoholditsruleandprovidebasisforartificialsexreversalDuringtheperiodfromJune2002toDece

6、mber2003，experimentsweredonetoinducesexreversalinordertoprobeintothefeasibilityinsexreversalandreproducingoffspringinsilvercarpDuringtheperiodfromDecember2002tOApril2003，RAPDtechniqueWasusedtoanalyzegeneticdiversityofinb

7、reedingFIprogenyintwoartificialgynogeneticstrainsofsilvercarp，inordertotestthestabifityoftheirgeneticcharactersThemainresultsobtaineda托USfollows：1GonadaldifferentiationofinbreedingFliIlgynogeneticstrainIofsilvercarpappea

8、redatanatomicallevelafterhatchingfor35days，andovarydifferentiatedfirstwiththeemergencyofovariancavityasamarkerThecrosssectionofovarywasstripshaped，andthatofspermarywasellipseAfterhatchingforthleemonths，thepositionofmainb

9、loodvesselinthegonadcouldalsobeusedasananatomicalmarkerforearlygonadaldifferentiationofsilvercarp，ofwhichthemainbloodvesseloftheovaryclosedwitllcenterofventralsideandthatofthespermaryclosedwithdorsalsideItwasdifficulttod

10、istinguishoogoninmfromspermatogoniumaccordingtotheshapeandsizeofgoniumunderlightmicroscopeAfterfivemonths，theovarystartedtoformaspawnstoredplate，andexistedinstageITheovaryofsevenmonthshadformedafullspawnstoredplateendexi