農(nóng)桿菌介導(dǎo)的Vf基因轉(zhuǎn)化蘋果的研究.pdf

1、r906口3lH女UDC菜陽農(nóng)學(xué)院碩士學(xué)位論文農(nóng)桿菌介導(dǎo)的vf基因轉(zhuǎn)化蘋果的研究smdy0fAgrD占acfe—um—mediatedTransforma十ion0fVfGeneinApple研究生姓名鞋師專業(yè)研究方向王恒振祝軍教授果樹學(xué)生物技術(shù)十目音島20066月萊陽農(nóng)學(xué)院碩=學(xué)位論文摘要Studyof彳g加6口c紀(jì)砌刀z—mediated11ransformationof侈GeneinAppleAbstractApplescabis

2、oneofthenlaindiseasesinIheapplepmductionre百onallaroundtheworldAtthemoment，themainapproachtoobtaintheresistanceculIivararcslillthetraditionalcrossbreedingmethodwhichistimeconsumingand10we師ciencyBasedontransfbmngIhe可gene，w

3、hichisreguIatedbyCaMVpmmoterandresjstanttoscab，into印ple，“ispossibkforustoachieVenewresistancecultivarsandacceleratelheprocessofscabresisIancebreediIlgInlhisstudy，high仔equencyregenemtionandlransfonnationsystemshavebeenest

4、ablished婦Dughselectingmebestconditionofpr01ifjration，regeneration，transfo腫aIionandantibioticselectionof印pk1eaves加vf肋，and可genehasbeeninsertedintonewcultivar‘FuzaohongThemainresultswereasfollows1Get娃ngmebestpmliferationcon

5、ditionofthreeapplecultivarsTheresearchindicaCedthatmetllreecultjvarsallhadgoodproliferationabilityand山eircul眥condi【ionsweret11efollowing3aspects：culturctemperarurewas25士2℃，illuminationdegreewas20003000Lxandmuminationtime

6、、粗s16“d2Gainingahighemcientandstableregenerationsystemof‘Fuzaobong’appleE膿tsofgenotype，ho珊onecombi眥tion，darkculturecjme，andAgN03werestudied，andahi曲efficientandstableregenerationsystemof’Fuzaohong’applewasde矗nedOnthemodi6

7、edMSmedi啪containingBA4orTl∥LandNAAO2mg／L，wifh15ddarkcultLlrethentransferredtolight，regenera石onbudsandshootswereobtained，andregeneralionrateof‘Fuzaohong’apple1eafreached9167％Atthesametime，difrerenthormonecombinationsofIAA

8、andIBAwerestLldied，themedium1，2MSIAAl5m∥L十IBAmg／Lwasgoodfor‘FuZaohong’，becausegmwjnginmismedium，ithadmanylongerrootandhadahi曲rooting丘equencyof95％3Establishinghi曲transfonnationfrequencysystemof‘Fuzaohong’andobtainingtrans