沙地柏生物生態(tài)因子與天然藥用產(chǎn)物鬼臼毒素含量關(guān)系研究.pdf

1、西北農(nóng)林科技大學(xué)碩士學(xué)位論文沙地柏生物生態(tài)因子與天然藥用產(chǎn)物鬼臼毒素含量關(guān)系研究姓名：程俊俠申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生態(tài)學(xué)指導(dǎo)教師：陳存根20050601THERELATIoNSHIPBETWEENTHEBIOTICANDECoLoGICALFACToRSANDPoDoPHYLLoToⅪNCoNTENTIN翩最日Ⅵ4眥G重弘S4ⅣZABSTRACTTheevergreenprostrateshrubSabinavulgarisAnt。(

2、syn：JuniperussabinaLinnaeus)belongingtothegenusSabina(familyCupressaceae)iswidelydistributedinthenorthwestofChinaMuchattentionhasbeenpaidtochemicalconstituents，bioactives，biotic，ecologicalandphysiologicalcharacteristicso

3、fthisplantOnetypeofmainlysecondarymetabolitesoftheplant，podophyllotoxinanditscongenersexhibitpronouncedbiologicalactivitymainlyasstrongantiviralagents，antineoplasticdrugs，andasinsecticidesHowevertheinfluencesofontogenyan

4、denvironmentonthecontentofpodophyrllotoxininSvulgarisstillremainunclearInordertoprovidscientificbasisforthefurtherrationalutilizationoftheSvulgarandpodophyllotoxin，therefore，inthispapertherelationshipbetweenthebioticande

5、cologicalfactorsandthepodophyllotoxincontentintheplantwerestudied，usingsupercriticalfluidextraction(SFE)andhiglperformancechromatography(HPLC)，andtheresultsweredescribedasfollowing：1Theconditionsforextractionofpodophyllo

6、toxinfromSvulgariswithSFEwerestudiedThepodophyllotoxincontentWaSanalyzedbyHPLCwithPADdetectorTheextractingefficiencywasevaluatedby2indexes，ie，totalcompoundextractionratioandpodophyllotoxinextractionratioUsingstaticextrac

7、tionfollowedbydynamicextractionmethods，theeffectsofdifferentparameters，suchaSpressure，temperature，modifiervolumeandextractiontime：el|一lb艫餓孽脯矗垃跳l|fluidextraction(SFE)of。礎(chǔ)plI毋lobDx蛹。州ereinvestigatedTheresultsshowedthat，und

8、erapressureof6000psi，temperature35“C，flowrateofC0240mL／g，andstaticextractiontimeof15min，extractionWasmoreselectiveforthepodophyllotoxin2Thepodophyllotoxincontentof＆vutgarisleavesandstemsin7differenthabitatswereexaminedTh

9、eresultsshowedthatthehabitatsexhibitedsignificantinfluencesonthecontentofpodophyllotoxinintheleaves，whilelittleinfluencesonthecontentofpodophyllotoxininthestems，andthecontentofthepodophyllotoxinoftheplantwasmorehigherinp

10、rotrudinglandswhilelesslowerinartificialwoods3Themineralsubstancesinsoilofsettledsands，protrudinglandandartificialwoodsweresurveyedrespectivelyandthereWasastronglinearrelationshipbetweennitrogenandsoilavailablenitrogenan