馬尾松兩系雜交F1代RAPD分子鑒別.pdf

1、中南林學(xué)院碩士學(xué)位論文馬尾松兩系雜交F1代RAPD分子鑒別姓名：申響保申請學(xué)位級別：碩士專業(yè)：林木遺傳育種指導(dǎo)教師：徐剛標(biāo)陳建華20050601碩士學(xué)位論文馬尾松兩系雜交Fl代RAPD分子鑒別ABSTRACTPinus9∞ssonJsnaisbelongedtoPJ力aceaoandPinusLItissoutheastwetnesssubtropicstheregiondistributemostwidelyinourcountry，

2、theresourceskeepsthemostabundantforestofquantitytype，alsoisthespecialimportantindustryrawmaterialinthesouthernofourcountryThereforechoosingthePjflusmassonianatreetogrowtheconductandactionshereditytheresourcesadvantagedep

3、thdiscorerwithhighhereditygoodkindofthequa]itywoodseedtoselectfortheobjectsofresearch，matchcompletelycurrentandfutureforestryproductthedevelopmentdemandandtheforestryscienceandtechnologyrequestoftheeconomicdevelopmentPin

4、usmasson]aflaloosenstodistributetheareainsidehavenumerousriverswithbighighmountains，thegeographicalenvironmentconditionvariety，becauseofthe10ng—termnaturechoiceandgrowtoinsulatetowaitthereason，existextremelyabundanthered

5、ityvariation，thismaterialhereditythatloosensforPinusmassonianatheimprovementprovJdedabundantmaterialfoundationThemainpurposeofthisresearchistomakeuseoftheloosedelicateleafofPinusmassoBianaasmaterial，toexamthefirstbreedin

6、gbetweenPinusmassonJanatheNo8LinduoandtheNo111ofGuangxiaremiscell—aneoustohandovertheuniformoftheprosterity，toadoptnewmethodexamingtheDNAsequence，carryonthePinusmassonianalooseaRAPDexpandstabletotaketheanalysisandcarryon

7、theRAPDmoleculermarkers，authenticatingtheproductionapplicationinthecharacteristicRAPDmoleculermarkersofaF1generation：InordertoopentheloosegeneticengineeringdefinitedirectionoftheexhibitionPinuSmassonianatogrowtolaythedep

8、endablefoundation、forthepurposeoffurtherresearchandinvestigatethePinusmassoniana，thelooseDNAforexpressdiscriminatedthetechniqueinearlydays，shortenningtogrowtheperiod，providingaqua]ityofhighgenerationfortheproduction，prod