低分子抑瘤物的分離鑒定及其誘導(dǎo)腫瘤細胞凋亡的研究.pdf

1、南開大學(xué)碩士學(xué)位論文低分子抑瘤物的分離鑒定及其誘導(dǎo)腫瘤細胞凋亡的研究姓名：徐暢申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：張金紅2001.5.1南開大學(xué)碩士研究生畢業(yè)(學(xué)位)論文ABSTRACTPreviousstudieshaveshowedthatthereareIOWmolecularweightnaturaltumorsuppressorsinthecrudeextractionofnewborncalfliver，w

2、hichhaspreferentialinhibitoryeffectonthegrowthofhumanleukemiccelllinesandexhibitsgoodpurgingeffectintheautologousbonemarrowtransplantationtOtreatmyeloidleukemiaFromthecrudeextractionofnew—borncalfliverwiththecharacterist

3、icsof10Wmolecularwei曲t(1essthan1OKDa)，wehaveisolatedfourlowmolecularweightnaturaltumorsuppressorsthroughionexchangechromatographyandadsorptionchromatographyOneofthemwasidentifiedtobeaknowncompound————tyramineandtheidenti

4、ficationoftheothercompoundsisinprogressWestudiedandcomparedtheeriectsofdifferentconcentrationsoftyramineor／anddibutylphthalate(DBP)ontheproliferationofhumanbreastadenocarcinomaMCF一7cellsResultsshowthatthetwoabovecompound

5、sbothinhibitedthegrowthofMCF7cellsandthecelldeathcausedbytyramineandDBPwasduetOapoptosisFurthmore，theantimmoractivitywasincreasedsignificantlywhenMCF7cellstreatedwiththecombinationoftyramineandDBEWiththeexposureofMCF一7ce

6、llstOtyramineandDBPDNAdegradedinacertainwayandproduced3050kbDNAfragmentsonagarosegelelectrophoresis，whichmaybeaphenomenonofapoptosisInaddition，theresultsofdotblottingdemonstratedthatmRNAlevelofp53wasgenerallyincreasedand

7、thatofbcl2wasdownregulatedaftertheincubationoftyramineandDBEThisimplicatedtheapoptosisinducedbytyramineandDBPcouldberelatedtotheregulationoftranscriptionofp53andbcl2Keywords：lowmolecularweightnaturaltumorsuppressortumorc