As-,2-O-,3-磁性納米微球聯(lián)合磁流體熱療靶向治療宮頸癌的研究.pdf

1、東南大學碩士學位論文AsO磁性納米微球聯(lián)合磁流體熱療靶向治療宮頸癌的研究姓名：賈秀鵬申請學位級別：碩士專業(yè)：病理學與病理生理學指導教師：張東生鄭杰20030613東南大學碩士學位論文AbstractTargetTherapeuticEffectofMagneticNanomicrospheresContainingAsz03inCombinationwithMagneticFluidHyperthermia(MFH)onCervical

2、CancerGraduate：JIAXiupengSupervisor：Pro，ZHANGDongshengProZHENGJieDepartmentofPathologyandPathophysiologySchoolofBasicMedicalScience，SoutheastUnivemityObjeetive①TostudythepreparationandcharactersofMnl刪ZnFe204(Mn—Znferrite

3、)(2)Tostudythepreparationandcharactersofmagneticnano—microspherescontainingAs203(9Tostudytheantitumoreffectsofmagneticnano—microspherescontainingAsz03inthehighfrequencyelectromagneticfieldoncervicalcanl：erinvitroandinviv

4、o，Method①Themagneticnanoparticles(MnlZnFe204)werepreparedbyimprovedco—precipitationmethodwhichcharacterizationwasevaluatedbyTEM、XRD、thermalanalysissystem②MagneticnanomicrospherescontainingAs203werepreparedbyamodifiedcold

5、—condensationmethod，whichcharacterizationmeasuredwithenergydispersivespectrometer、TEM、computercolormagicimageanalysissystem、atomicfluorescencespectrophotometry、atomicabsorptionspectrometryanddynamicdialysissystem③Invitro

6、antitumoreffectofmagneticnanomierospherescontainingAs203onSiHacellswereobservedbyM訂methodflowcytometryXenografltumorswereinducedinthesubcutaneoustissuearoundtherightshoulderbladeofnudemicewithSmacellsThreekindsofnanomicr

7、ospheres(nano—microspherescontainingAs203、nanomicrospherescontainingmagneticparticles、magneticnano—microspherescontainingAs203)wereinjectedintothetissueofnudemice，whereSiHacellshadbeentransplanted，Themiceofexperimentalgr

8、oupexploredtOhighfrequencyalternatingmagneticfieldforthreetimesThegrowthoftransplantedtumorsofnudemicewasmomitoredfor45dayscomparedwiththecontrolgroupResults03Theaveragediameterofmagneticparticleswereabout50nm，round，whic

9、hhadstrongmagneticresponsivenessItschartofXRDwasconsistentwiththestandarddatathemagneticparticlesofmicrospherecarrierTcwas1054。CWhileitsmagneticfluidcouldriseto43℃47℃duetodifferentconcentration②Magneticnano—microspheresc

10、ontainingAs203weresphericalwiththediameterof190nm_50nmAs203contentwas065989_2042#g／mgandMn—ZnFerrite6957027蝴36ug／mginnano—microspheresrespectively③Magneticnano—microspherescontainingAsz03hadstrongercytotoxitytoSiHacellsi

11、nvitroThecellsurvivalratiointhegroupofmagneticnano—microspherescontainingAs203underhighfrequencyalternatingelectromagneticfieldwerelower(4274％)thanthatofsingleAS203(7777％，P0001)，nonmagneticmicrospheres(6041％，PO01)andmagn

12、eticnanomierospheres(5726％，P0oDThegelatinandsinglemagneticnanomicrosphereshadnoeffectoncellgrowthInvivo，theinhibitoryeffectofthemagneticnano—microspherescontainingAs_203ontumorgrowthwasveryobviousfweightinhibitoryratio99