稻瘟病菌成熟附著胞cDNA差減文庫(kù)的構(gòu)建.pdf

1、≥之j1碩士學(xué)位論文稻瘟病菌成熟附著胞cDNA差減文庫(kù)的構(gòu)建作者姓名劉同寶指導(dǎo)教師林福呈教授盧建平副教授學(xué)科、專(zhuān)業(yè)所在學(xué)院提交日期微生物學(xué)生命科學(xué)學(xué)院二零零ifi年丘月中國(guó)杭』1’lTheConstructionofcDNASubtractionLibrariesinAssociationWithMatureAppressoriumofMagnaportheAbstractTheappressoriumisaspecializedcel

2、lularstructureformedbyMagnaporthegriseabeforepenetratingplantissueThisappressofiurnstageisakeyperiodformericeblastfungustoinfectriceItissignificanttofmdoutthestageofgeneexpressionofappressoriumforUStounderstandtheprocess

3、ofappressoriumformation。developmentandpenetrationinthericeblastfungusInthisarticle，wereportedthediscoveryoftranscriptsuniquelyexpressedduringlateappressoriumstagein膨griseabyasuppressionsubtracfivehybridizationmethod，andt

4、riedtofindthekeygenerelatedtoappressoriumformation，developmentandpenetrmionTheresultsobtainedareflsfollowing：TheexperimentwascarriedOUtwithappressoriuminduced0nprojectionmembraneandmixtureofmyceliumandcouldiacontr01Asthe

5、conidialconcentrationwas1X106mrl，theratioofappressoriumformationonthehydrophobicsurfaceofprojectionmembraneWas96。5％24hoursaftereonidiainoculatedTo“RNAWasextractedfromappressofiumandmixtureofmycefiumandcorddiaandtheconcen

6、trationwas14299／“l(fā)and509牡g仙IrespectivelyTheRNAofappressoriumandmixatreofmyceliumandconidiaWasreversetranscriptedintoeDNAbysmartskillandlongdistancePCRskillThelengthoftwokindsofeDNAwasbetween200bpand6KbThelengthofappresso

7、riumcDNAWasmainlybetween500bpand5kbandthatofdrivereDNAwasmainlybetween400bpand2kbAsubtractivesuppressivelibraryofmatureappressoriumeDNAsubtractedbyconidiaandmyceliumcDNAwereconstructedbySSH(Suppressionsubtractivehybridiz