陰道加德納菌幾種檢測方法對細(xì)菌性陰道病診斷價值評價.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文陰道加德納菌幾種檢測方法對細(xì)菌性陰道病診斷價值評價姓名：劉俊芬申請學(xué)位級別：碩士專業(yè)：微生物與免疫指導(dǎo)教師：李連青20040526Abstract0bjectiveToevaluatethedetectionmethodsofgardnerellavaginalis(GV、inlaboratoryastothediagnosiSofbacterialvaginosisandtoprovidethefoundati

2、onofthebacterialvaginosis(BV)indiagnosisToexaminedmgsensitivitiesofGVtoseveralclinicaltraditionaldrugsandtogivetheclinicaldoctorsadvicesmatwhichdrugshouldbeselectedMethodsTousepolymerasechainreaction(PCR)methodtodetectga

3、rduerellavaginalis’SspecificgenefragmentGramsstainingandbacterialculturewerealsousedtodetectGVAndarapidlydiagnosistestofBVwereusedtodetecttheproductofBVResultsTherewere237samplesofvaginalsecretiontobeexaminedintotalAccor

4、dingtothegoldstandard(Amselstandard)，therewere174casesinBVgroup，63casesinnonbacterialvaginosis(NBV)groupand40personsinhealthycontrolgroupThepositiveratesofthefourlaboratorymethodsofGVweresignificantlyhigherinBVgroupthant

5、hoseinNBVgroupandthoseinhealthycontrolgroup(X2=1472，3337，8689，8843and1617，1764，8407，13232，respectively，P005)InGramsstainingthereweredifferenceofthepositiveratesofGV，CluecellandWBCinstatisticsbetweenBVgroupandNBVgroup(X2=

6、6538，4491，950，respectivelyP005)23isolatesofGVwereexaminedforsensitivitiestometronidazole，tinidazole，clindamycin，azithromycin，roxithromycinTherewere2isolatesofGVtobesensitivetometronidazole3isolatesofGVtobesensitivetotini

7、dazole，3isolatesofGVtobesensitivetoxithromyein，4isolatesofGVtobesensitivetoazithromycinand1isolatesofGVtobesensitivetoclindamycinExceptforthoseisolatesofGVabove，tlleywereallresistanttothetraditionaldrugsConclusionGVplays

8、animportantroleinmicrobiotaofBVbecauseitconsistsof113～1／2microbiotaofBVTherapiddiagnosistestofbacterialvaginosisisarapidandgoodsensitivitymethodthatisfitforthescreeningofthecrowdGramsstainingisasimplemethodwhichcouldbeea