淋巴瘤B細(xì)胞多藥耐藥細(xì)胞系的建立及耐藥機(jī)制研究.pdf

1、南開(kāi)大學(xué)碩士學(xué)位論文淋巴瘤B細(xì)胞多藥耐藥細(xì)胞系的建立及耐藥機(jī)制研究姓名：蘇改秀申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：臨床醫(yī)學(xué)指導(dǎo)教師：楊純正200405012004屆南開(kāi)大學(xué)醫(yī)學(xué)院七年制臨床醫(yī)學(xué)專業(yè)碩士畢業(yè)論文EstablishmentofBcellmultidrugresistancemodelinlymphomaanditsmolecularmechanismsDegreeCandidate：SuGalXiuTutor：YangChunZheng

2、Abstractobjectives：ToestablishamultidrugresistancecelllineofRajiandinvestigatepossiblemechanismsofacquiredresistanceMethods：HumanlymphomaRajicellswereculturedingraduallyinerensingconcentrationofDoxorubieinov盯halfayeartog

3、aintheresistancelineMTTandRTPCRwereusedtostudythepossiblemechanismsoftheresistanceResults：WehaveestablishedamultidrugresistancecelllineofRajiwimabout10一如ldresitancetoDoxorubicincompared岍Ⅱlthecorrespondingsensitivecelllin

4、eOtheTIllore，wehavefoundabroadspectrumofdrugresistancetootheranticanceragentssuchasHHTVPl6，VCRandDDPIntermsofthecellappearanceandtheproliferation，ithasnoobviousdifferencebt!tweantheresistanceandsensitiveeelllineTheexpres

5、sionleveloftheImpandbcl2genehasinereasedclearlyinresistancecelllinebutmdrlhasnochangeWheatreatedwithantiCD20monoeloneantiboaybcl2hasnoobviouschangeConclusions：AmultidrugresistancecelllineofRajiWasestablishedThemainmolecu

6、larmechanismsofthedrugresistanceinvolvedincreasedexpressionofImpandbcl一2TheexpressinglevelofmdrlhadnOchangeVl／hantreatedwithanti—CD20monocloneantibodybel2hasnoobviouschangeItselfisthattheantibodyaffectsmultidrugresistant