姜黃素對(duì)紫杉醇誘導(dǎo)的人卵巢癌細(xì)胞survivin、Bcl-2表達(dá)的影響.pdf

1、學(xué)位論文姜黃素對(duì)紫杉醇誘導(dǎo)的人卵巢癌細(xì)胞survl—vln、Bcl2表達(dá)的影響CurcuminEffectstheExpressionofsurvivin、Bcl一2InducedbyPaclitaxelinOvarianCancerCells申請(qǐng)學(xué)位級(jí)別：亟專業(yè)名稱：蟲醫(yī)妲型堂論文提交時(shí)間：2Q12生壘旦論文答辯時(shí)間：2Q12生旦二。一二年四月成都中醫(yī)藥大學(xué)碩士研究生畢業(yè)論文AbstractObjective：Toinvetstthe

2、expressionlevelofsurvivinorBcl一2proteininducedbypaclitaxelandcellsurvivalrateinhumanovariancancercellSKOV3(MyD88)，A2780(MyD88)，whichCanrevealthemechanismwhichcurcuminsensitizedpaclitaxelchemotherapyMethods：(1)ELISAmethod

3、wasusedtodetecttheexpressionlevelofsurvivinorBcl2inovariancancercells(2)Bradfordassayforthedetectionoftotalproteinexpression，andPg／mgproteinshowedtheexpressionlevelofthetwotargetproteins(3)MTTmethodwasusedtodetectetheova

4、riancancercellgrowthinhibitionbydrugsResults：(1)PaclitaxelandLPSCanpromotetheexpressionofsurvivinandBcl2onconcentrationandtimedependentmannerintheSKOV3cells，paclitaxelcoulddecreasetheexpressionoftwotargetproteinsinA2780c

5、ells，However，LPScouldnot(2)Curcumincouldsignificantlydecreasetheexpressionoftwotargetproteinsathighconcentrationsinthebothcelllines，However，thelowconcentrationofthedrugcouldnot(3)Comparedwiththedrugalone，combinedwithcurc

6、uminonhi曲concentrationstheexpressionofsurvivinandBcl一2couldbesignificantlydecreased，HoweverthelowonescombinedwithpaclitaxelorLPSonlydecreasedsurvivinproteintheexpression，notBcl2(4)PaclitaxelandcurcuminCaninhibittheprolif

7、erationofSKOV3andA2780cellsanddecreasetheIC50，coadministrationwitheachothercouldsignificantlystrengthentheeffectConclusions：(1)InMyD88ovariancancercell，paclitaxelTLR4／MyD88surviving／Bcl2signalingpathwayisexisted(2)Paclit

8、axelorLPScouldactivatethewaytopromotetheoverexpressionofsurvivin／Bcl一2，howevercurcumincouldsuppressitintimeandconcentrationdependentmailer，decresingtheexpressionofthetwoproteins，especiallyinMyD88ovariancancercelllines(3)