富有柿與次郎柿組織培養(yǎng)技術的研究.pdf

1、河北農(nóng)業(yè)大學碩士學位論文富有柿與次郎柿組織培養(yǎng)技術的研究姓名：劉愷申請學位級別：碩士專業(yè)：農(nóng)產(chǎn)品加工及貯藏工程指導教師：馬俊蓮張子德20050616StudyonTissueCultureof‘Fuyu’andJiro’Persimmon(DiospyroskakiThunb)Author：LiuKaiSubject：FoodPIxⅪessandStorage輕晦nB疆嫩gSupervisor：MaJunlianZhangZideAbs

2、tractDiospyroska艇ThunbcvFuyuandJiroaleJapanesepersimmonvarietiesDormantbudstakenfrompersimmontrees，asinvitroexplantsmaterials，weresetupsuccessfullyNewshoots黼attainede粗lUSWaSinducedandadventitiousbudsweredifferentiatedfro

3、mleavesandrootsoftissuecultures；newshootspropagationandshootsroofingwerestudiedindifferentbasalmediumaddeddifferenthormones。ThepaperCouldbeusefullformodifiedgenepersimmonandforfastpropagationplantsaswellResults囂sfollowin

4、g：iMicropropagationoftwopersimmonverifieswasconductedEffectsofbasalmediumsandhormones011thepropagationw懿studiedbyOrthogonaldesigntrialResultsshowedD|哪mediumwasthebestbasalmediumandMSwasmuchbetterthanWPMwhichcouldnotbeuse

5、dforthetWOpersimmonpropagation。‘&弦persimmoninZT10瓣g甩withoutBAmediumgrewbestThereWasmuchmorelongershoots詛甜20meJLforJim’，butcallusWaslargerwhichWasnotbeneficialtothe蜮國吶雌在onDKWmediumconminingZT10mg／LandBA05攮班一Wassuitablefor

6、the‘Jiro’micmpropergation。；2Ef艷ctsofbasalmediumshormonesandtheirconcentrationsonshootsregenerationfromleavesof‘翔yll’and‘Jiro’werestudiedTDZwasfoundedtobeproperforbuddifferentiation，thebestshootbudsfromleavesof‘Fu粥’and‘Ji

7、ro’inductionmediawast／2DKW7IDZO5擔g／L。After30days，theealliWeresubdividedandtransferredtoI／2DKWmediumsupplementedwith10mg／LZTand01mg／LIAAAfter30days，regenerationrateexceeded90％andaverageNoofbudofeachexptantwithbudsexceed7。

8、8。56respectively3Newshootsweredifferentiatedfromrootsof‘Fuyu’andJiro’shootsafter20dayssincecultureinthemediumcontainingZTandaveragenoofshootsWaS67，63respectively4Whensubeulturetimesislessthan15，1／2MSmediumsuitsrootingof‘

9、FUyu’and‘Jiro’persimmon。I／2MSandlt2MS(1／2N)mediumbothsuitrootingfrom‘№”and‘Jiro’shootaftersubculturetimesismorethan15IBAWaSmoreeffectivethanIAA。Shootscultured如mediumcontainingIBA10啦andexceeding90percentofshootsarerooted5