培養(yǎng)大腦皮層感覺(jué)運(yùn)動(dòng)神經(jīng)元活動(dòng)依賴突觸可塑性機(jī)制的研究.pdf

1、Postdoctoralr印砸ofAca蛐ofMmta】了Medic“Sciencesensorimotorneuronsdllr血gmec咖lperiodshowedtllatthedis仃ibutionwasskewedtowardlargereventsthatindic砒esatypicalglutamatergicdistributionhlt11erepetitivestimulationex耐腓吣，voltagepulse

2、successmllyillducedsyTlapticIesponseofsEPSCs蛐d吐圮e疵ctw髂hi曲lyreproducible(95％，n=12)Pfedominantly，tllerapidresponseincreasedsignificantlyj且theaveraged舶quency(帥icallyaID啪d2—4folds)accomp肌yingachallgeiIlaveraged鋤pl如de，ofsEPSC

3、s111eellllallcedEPSCs舶quencyresponsegenemllyma灑ta證ed塒t11iIl30—40miIloccurringiIlmostcellsIIIadditionVoltagepulseinducedpotentiational∞in、，olvedincre船eillspike丘equencyinsomeneurons(around8一lOfolds)Toex御ninethemechaIli鋤ofi

4、IlductionandtheexpressionofEPSCs舶qu∞cyfesl)0璐e，“appcared，岫defconditionsofourexperiments，toimpIya11證crca∞ofpnsymIpticse衄iti、，itymatmaybedueinpan，tothetIⅡnjngonofsnentsynapses五0wwer’tllechan伊ofEPSCsarnplitudeiIldicatingtll

5、iss、時(shí)cchmightmnect鋤inc他鵲ei11也epostsyn印ticsensitiVityofnon廿∞AorNMDArec印咕orsatp咒viouSlyactivesynapses，oraltematiVely’itcouldrenectthetumingonoffele雒esitestllatwere丘mctionaUysilentpriortothevol詛gepllisillgNev鋤el媯s，wec姐notex

6、cludethepHDssibil竹thatsome缸ctionofpot蹦tialionofEPSCsispostsyn印ticWhichrequires姐increaseiIlpostsynapticCa2Wi也∞specttospiI【e五requencyresponsc，曲呤possiblemechaIlismmi曲tbeinvolved姐aJte枷0nof也evoltage—gatedNachannels吐’atmayacco

7、untforthech鋤geillsyIlapticstrcngtllhlagrcementofrecentstudiesofcuh聃dhippocampalnemons，VDltagepulse—inducedpotentiationincultu∞dcomcalsensorimotorneuronsistraIlsientsuggestiIlgtllatsus扭iIledpotentiationmayrequiretllecombm