皮下注射硝酸甘油誘導(dǎo)的偏頭痛大鼠三叉神經(jīng)尾側(cè)核NF-KappaB的表達(dá)研究.pdf

1、分類號密級Y902956單位代碼：10114學(xué)號：2004286皮下注射硝酸甘油誘導(dǎo)的偏頭痛大鼠三叉神經(jīng)尾側(cè)核NFKappaB的表達(dá)研究研究生：楊廈自指導(dǎo)教師：申請學(xué)位門類級別：專業(yè)名稱；研究方向：所在學(xué)院：生生王熬撞亟盈絲通堂垣迭猛山酉醫(yī)型盍堂筮二蝤壓醫(yī)生瞳2006年4月20口山西醫(yī)科大學(xué)碩士學(xué)位論ThestudyofNFKappaBexpressionintrigeminalnervecaudalcoreofthemigrainin

2、gratsinducedwithGiyceryltrinitratesubcutaneousinjectedAbstractObject：ToexploretheeffectofNFKappaBintheonsetofmigrainebytheobservationofNF—KappaBexpressionintrigeminalnervecaudalcoreofthemigrainingrats，Methods：Todividethe

3、researchtotwopartsInthefirstpart，72Wisterratswererandomallocatedinto6experimentalgroupsand6controlgroups(6rats／group)Glyceryltrinitrate(OTN)andisotonicNachlorideweresubcutaneousinjectedintotheexperimentalgroupsandcontrol

4、groupsrespectivelyAfter1，2，3，4，5，6hours，thedistributionofNFKappaBpositivestainingcellsinthetrigeminalnervecaudalcoreofthemigrainingratsweredeterminedwithimmunohistochemistryInthesecondpart，usingWesternblotanalyticalmetho

5、dtOsemiquantitativeanalysetheexpressionofNFKappaBproteinumintrigeminalnervecaudalcoreofthemigrainingratsinthecorrespondingtimesofthefristpartResults：ImmunohistochemistrydyeingdiscoverallofthecontrolgroupshavetheNFKappaBn

6、uclearpositivereactionincaudalcoreoftrigeminalnerve，andthereisnoobviousdifferencebetweeneachvarioustimegroup，doesnothavestatisticssignificance(P005)；theNFKappaBnuclearpositivereactioninallexperimentalgroupsincreasesmoret

7、hancontrolgroups，otherwisewithintheexperimentalgroup，activationofNF—KappaBat3rdand4thhourismorethan1，2，5，6hoursgroups，havestatisticssignificance(P005)TheWestemblotanalysisconfirmsthesameruleofNF—KappaBproteinexpression；3

8、hoursgroupshavestatisticssignificancewithothereachgroupofcomparisons(Po05)，while2，3，4hoursgroupsaremorethan1，5，6hoursgroups(PO05)Conclusions：TheexpressionofNFKappaBproteinumintrigeminalnervecaudalcoreofratswasreinforceda