中藥抗真菌活性測定和酵母利用非發(fā)酵碳源基因的篩選.pdf

1、天津大學(xué)碩士學(xué)位論文中藥抗真菌活性測定和酵母利用非發(fā)酵碳源基因的篩選姓名：趙炯申請學(xué)位級別：碩士專業(yè)：藥物化學(xué)指導(dǎo)教師：蔣伶活20070101ABSTRACTPROJECTONE：Object：Toassessthereliabilityofbothdryandwetdiskdiffusionmethodsinantifungalactivityassayofextracts舶mChinesetraditionalherbsMethod

2、s：Candidaalbicans，themostimportamhumanfungalpathogenwasusedasthetestorganismThedryandwetdiskdiffusionmethodswereusedtoassaytheantiCandidaalbicansactivityofextractsofChinesetraditiomlherbsandplantsBothmethodshavedemonstra

3、tedgoodconsistencywiththeformula∥=AbgQ必whichrepresentsthatthediagramsquareofinhibitionzoneisindirectratiotothelogarithmofdrugcontentThereisnosignificantdifferencebetweenthetwomethodsWeexaminedtheantiCandidaactivitiesofet

4、hanolextractsof27Chinesetraditionalherbsandoilextractsof29speciesofplantsbybothmethodsTheresultsshowthatThamnoliavermicularis，RhizormaParidisandoilextractsofcinmmoncontaininhibitorycompoundsagainstCandidaalbicansConsider

5、ingvarioussolubilityofherbsextracts，thewetdiskdiffusionmethodwouldbecomplementarytothedD，methodinas∞ssingtheantifungalactivitiesPROJECTTwO：Object：Toscreengenemutationshavingdefectsinutilizationofnonfermentablecarbonsourc

6、esinSaccharomycescerevisiaeMethods：ReplicatingthegenedeletionstrainlibraryofSaccharomycescerevisiaeolⅢioYPD(2％ghcosc)，YPE(2％ethan01)andYPG(2％glycer01)platesExamininggrowthdefectsofstrainsonYPEandYPGincomparisontoYPDConfi

7、rmationofgrowthdefectswereassayedbyserialdilutionsResults：172strainsshowinggrowthdefectsonbothYPEandYPG，16strainsonlyonYPG，and35strainsonlyonYPEaloneComlusion：identificationofgenemutationsinutilizationofnonfermentablecar

8、bonsourceswouHprovidecriticalinformationforelucidationofca而onmetabolismsinSaccharomycescerevisiaeKEYWORDS：diskdiffusionmethod；Candidaalbicans；Chine∞traditionalherb；antifungalactivity；Saccharomycescerevisiae；nonfermentabl