貴州省部分主要甘藍型油菜雜交種及其親本指紋圖譜構(gòu)建與純度鑒定.pdf

1、貴州大學碩士學位論文貴州省部分主要甘藍型油菜雜交種及其親本指紋圖譜構(gòu)建與純度鑒定姓名：馬曉峰申請學位級別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：王通強20060501基捌太堂亟主堂焦迨塞蚩趔笪整僉主要苴墮型迪墓盤鑾弛厘甚塞奎i旨筮圖譜掏建皇綞廑鑒定AbstractInthisstudy，morphologymethodandtheSSRmarkermethodwereusedtoanalyze43brassicanapusmaterialsi

2、ncludingguizaserieshybridandtheirparentsetcDifferencesbetweenthetwomethodwerediscussedHighmulti—statepropertySSRprimerwereselectedandusedinbuildingDNAfingerprintingdatabaseofguizaserieshy梳dandtheirparentsThemainresultswe

3、reasfollows：1Morphologymethodwereusuallyusedtoobservecharacteristicofbrassicanapushybridsandtheirparentsinseedidentificationandpuritybutthismethodisnotexact224bmssicanapusinbredlineswereanalyzedbyusingSSRtechnicsTwenty—o

4、nepairsoutofonehundredpairsofSSRprimerswereselectedtogavepolymorphic，stableandrepeatedamplificationprofilesFourprimersincludingNal2C06、Nal0804b、0110A05、Nal0D03wereselectedtobuildtheDNAfingerprintingdatabaseofall24inbredl

5、ines3DNAfingerprintmapof19brassicanapushybridswerebuiltwith21pairsSSRprimersTheseprimersalsogavepolymorphic，stableandrepeatedamplificationprofilesFourprimersincludingNal2C06、Nal0804b、0110A05、Nal0D03wereselectedtobuildthe

6、DNAfingerprintingdatabaseofall19brassicanapushybrids4TestinthisresearchprovedthatSSRtechnicsCanbeusedinidentifyingthepurityandrealityofbrassicanapushybridsquicklyandprecisely5UseingprimeringroupmethodCanexpandthecoreprim