小麥成熟胚轉(zhuǎn)化系統(tǒng)的建立與轉(zhuǎn)鹽脅迫應(yīng)答基因植株的獲得.pdf

1、Y958918廿炎吼—079507—860789四川農(nóng)業(yè)大學(xué)碩士學(xué)位論文小麥成熟胚轉(zhuǎn)化系統(tǒng)的建立與轉(zhuǎn)鹽脅追應(yīng)答基因檀株的獲得指導(dǎo)教師學(xué)位類別領(lǐng)域名稱研究方向吳峰韭垂鹺一熬撞壁堂亟～生望絲堂童鹽王生塹芏應(yīng)田岔工生物蘭_一一2006年6月英文摘要Establishmentofembryogenictransformationsysteminwheatandproductionoftransgenicwheatwithsaltresponsi

2、vegene’WuFeng03iologyChemistryandMolecularBiology)，DirectedbyRenZhenglongAbstractMatureembryosofwheatvarietiesR57(ChuanNongl7)and／759(ChuanNongl2)whichareimportanteultivarinSichuanProvincewereusedasexplantsforcultureinvi

3、trotostudytheeffectsofconservationonratioofcallusinducedfrommaturewheatembryosInthisworkwealsesearchedtheeffectofindividualwechromosomeIRSOiltheinductionofcallusandtheregeneratingabilityofcallusculturesinculturedmatureem

4、bryosofwheatvarietiesR57、R59、R25(Chuaganongl鼢andwheatweIRS／1BLtranslocationlinesconstructedwithcommonwheatcultivarMYll(Mianyang11)andinbredwelinesR12，R3，L155and‘baili”ryeFurthermoreasaltresponsivegeneSadenosylmethionined

5、ecarboxylase(SAMDC)genewas缸ansformedtomatureembryosofwheatviamieroprojectilebombardmentSalttoleranceanalysiswasimplementedtoTtprogenyInthisresearchwestudiedthedifferentfactorsinfluencingthebiolisfiebombardmentinwheattran

6、sferandestablishedpreliminarywheatmatureembryoparticlebiolistictransformationsystem，MatureembryosofnewwheatvarietyR57andR59，whichhasbeenconservedfortwoyearswereusedasexplantforcultureinvitroTheresultshowsthattheratioofca

7、llusinducedofthe2yearconservedisgreatlydifferentfromthef托shmatureembryosFormatureembryosharvestedatdifferenttimeinthesameyearandhadthesameconservationtimethematureembryosharvestedlaterhadstrongerabilityofcallusformationt

8、hantheearly011eMatureembryosofwheatvarietiesedTR59，R25andthreewheatryetrans／oeationlineswhichbothhaveweIILSchromosomewe[eused雒explantsforcultureinvitrotostudytheeffectofwechromosomesOucallusinductionandregenerationincall