泥蚶凝集素的分離純化、理化性質(zhì)及血清的免疫因子研究.pdf

1、福建師范大學(xué)碩士學(xué)位論文泥蚶凝集素的分離純化、理化性質(zhì)及血清的免疫因子研究姓名：柯佳穎申請學(xué)位級別：碩士專業(yè)：動物學(xué)指導(dǎo)教師：陳寅山20060401塑壟塹翌盔蘭塑垡塑堡鱟生絲塞AbstractThehaemolymphandmuscleextractsfrom7speciesofbivalveWeretestedforagglutinatingactivityusing10kindsoferythroeytesofhumanandani

2、mal，8speciesofunicellularalgaeand10kindsofunicellularmicrobeTheresultsshowedth砒botllhaemolymphandmuscleextractsofbivalvecontainlectinsandtherewereevidentdifferencebetweenthemfromthesanlebivalve，anditisconferredthatthelec

3、tiusofhaemolymphandmuscleextractsfromthebivalveweredifferentintypesTheerythroeytesfromrabbit、Porphyridiumpurpureum，Saccharomycescarlsbergensisarethemostsensitivecellsrespectivelybysynthesizingtheagglutinationactivityofha

4、emolymphandmuscleextractsofbivalve。TakingTegillarcagranosa嬲experimentmaterial，byextractionfractionationwithsaturatedammoniumsulfateprecipitation(40％20％)，gelfiltrationontheSephadexG100andfollowedbyabsorptionchromatography

5、onSepharose4B，alectin(TGLh)waspurifiedfromthehemolymphofZgranosanlemusclelectinofZgranosa(TGLm、WaSpurifiedbyfractionationofhi曲concentratedammoniumsulfate(65％45％)，ionexchangechromatographyontheDEAESepharoseeolumandgelchro

6、matographyontheSephacrylS200HReolumFurthermorethemolecularweight、contentofneutralsaeeharide、pI、ultravioletabsorbingspectrum、aminoacidcompositionoftwokindsoflectinsweredetermined，anderythrocytesspecificity(nativeandenzyme

7、treatederythrocytes)、earbohydrate—bindingspecificity，thermostability，andinfluenceofpHandsalinity、13mercaptoethanol、bivalentmetalcationstotheagglutinativeactivitywerecomparedbetweenTGLhandTGLmTheybothhavetheabilityofmarke

8、dlypromotingthelymphocytetransformactionandtheactivityofdifferentantibacterial、antifongalThecrossreactivityofTGL—handTGLmwasanalyzedbydoubleimmunodifusiontestItWasfoundthattherabbitspecificanti辯lBmagainsteitherTGL—horTGL