圓葉牽?；ㄇ嗨卮x途徑的基因型變異與花色變異的進(jìn)化生物學(xué)研究.pdf

1、中國科學(xué)院植物研究所博士后學(xué)位論文圓葉牽牛花青素代謝途徑的基因型變異與花色變異的進(jìn)化生物學(xué)研究姓名：黃金霞申請學(xué)位級別：博士后專業(yè)：系統(tǒng)與進(jìn)化生物學(xué)指導(dǎo)教師：魯迎青20060701英文摘要HowisgenotypicvariationrelatedtothephenotypicvariationhasremainedoneofthefundamentalquestionsinbiologyOneeffectivewayofaddress

2、ingthisissueistoexploretherelationshipbetweenmolecularevolutionoffunctionalgenesandtheirphenotypiceffectsHereweutilizedanthocyaninpathway(whichdetermineswhatpigmentistobeproducedinflower)inthecommonmorningglory(Ipomoeapu

3、rpurea)tostudytwoquestions：1)whatisthebiologicalsignificanceofallelicpolymorphismand2)whatistheeffectofphenotypicselectiononthemolecularevoltationForthefirstquestion，weinvestigatedallelicpolymorphismatseverallociofthestr

4、ucturalandregulatorygenesaffectingtheanthocyaninpathwayincludingthoseencodingthefirstenzymeonthepathwaychalconesynthaseD(CHSD)，downstreamenzymesanthocyanidinsynthase(ANS)andUDPglucoseflavonoid3glucosyltransferase(UF3GT)，

5、andmybgenesattheregulatoryWlocusWethenconductedselfpollinationinthegreenhousetoobtainhomozygoteindividualsForthesecondquestionweinvestigatedthepollinatorsandflowervisitingfrequenciesofthepollinatorsonIpomoeapurpureainthe

6、fieldWethencomparedthegeneticvariationsbetweenwhite—floweredandcolorfloweredindividualsThecurrentdatashowthatthereareatleastthreeallelesofCHSDamongtheChinesepopulationsoneofthemisnewlyfound，inadditiontotheknown7allelesFi

7、vealleleswerediscoveredattheANSlocusandthreeofthemfirstlyobservedinChinaTwoknownalleleswerefoundattheUF3GTlocusThefunctionalalleleattheWlocusislpmyb2，whichwasnewlyfoundinChinesepopulations，anditdiffersmainlyintheintronre

8、gionsfromthoseofthereposedalleleIpmybl；incomparisontolpmyb2，thenewlyfoundsecondallele勿myb2haseightsubstitutionsintheexonsa6bpdeletion，anda19bpdeletion，causingORFshifting，andexistsmostlyinwhite—floweredindividualsThefield

9、observationsonthepollinatorsshowthatwhite—floweredplantsgetfewervisitsthancolorfloweredindividualsThepollinatorsonChineselpomoeapurpureaaremostlymoths(MacroglossumstellatarumL)ratherthanbumblebeesasfoundinAmerica，andthem