大蒜素體外抗白念珠菌生物膜作用的初步研究.pdf

1、華中科技大學(xué)碩士學(xué)位論文大蒜素體外抗白念珠菌生物膜作用的初步研究姓名：王煥麗申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：皮膚病與性病學(xué)指導(dǎo)教師：陳興平20090501華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文3InVitroActivityofAllicinagainstCidaalbicansBiofilmsAbstractObjective:ToinvestigatetheinvitroeffectsofallicinonCidaalbicans

2、biofilms.Methods:StardizedCidaalbicansstrainY01:09wasusedinthisstudy.MICofallicinfCidaalbicanswastestedbythebrothmicrodilutiontest.Cidaalbicansbiofilmswereculturedin96welltissuecultureplates.MTTreductionassaywasperfmedto

3、determinetheeffectsofallicinonCidaalbicansbiofilmsfmationcellsadherence.MicroscopicexaminationwasconductedtoassesstheeffectofallicinonmphogenesisofCidaalbicansbiofilmfmation.Serumgermtubecountingwasusedtoevaluatetheeffec

4、tofallicinongermtubefmationofCidaalbicans.MTTreductionassaywasusedtoevaluatetheeffectofcoated96welltissuecultureplateswithallicinonbiofilmsfmationofCidaalbicans.Cytotoxicassaywasusedtomeasuretheadverseeffectofallicin.Res

5、ults:MICofallicinfCidaalbicansis16ugml.AllicincaninhibitthebiofilmsfmationofCidaalbicansindoserelatedway.Allicinshowsdistinctbiofilmsinhibitioningrowthphasesofearly、intermediatematuration.Allicincaninhibittheadherenceger

6、mtubefmationofCidaalbicans.Coated96welltissuecultureplateswithhighconcentrationsallicincaninhibitthebiofilmsfmationofCidaalbicans.Allicinshowslesscytotoxicity.Conclusions:Allicindisplayspotentinvitroactivityagainstbiofil