卵巢顆粒細胞瘤中WT-1蛋白、AMH和Ki-67的表達及其意義的研究.pdf

1、山西醫(yī)科大學碩士學位論文卵巢顆粒細胞瘤中WT1蛋白、AMH和Ki67的表達及其意義的研究姓名：張盈盈申請學位級別：碩士專業(yè)：病理學與病理生理學指導教師：姚漢武20090315西醫(yī)科大學碩十學位論文ExpressionofWT1proteinAMHandKi一67inovariangranulosacelltumourandthesignificanceAbstractObjective：Todetecttheexpressionof、秤

2、1protein，Anti—MullerianHormoneandKi一67inovariangranulosacelltumourAndtoexploretheexpressionofWT1proteinandAntiMullerianHormoneinthedevelopmentofovariangranulosacelltumourandtherelationshipbetweenthemToexploretheroleofWT1

3、proteinandAnti—MullerianHormoneinthedevelopmentofovariangranulosacelltumourandtostudytherelationshipbetweentheexpressionofthemandthebionomicsofovariangranulosacelltumourBesides，wetrytoexplorethevalueoftumoursdifferentiat

4、ioninpredictingtheprognosisofovarianGeTMethods：37casesoftheovariangranulosacelltumourand10casesofnormalovaryweredetectedonTissueMicroarries，theexpressionofW，T1protein，AMHandKi67wasmeasuredwithlmmunohistchemistryTheresult

5、swereanalyzedwithSPSS11，5statisticalsoftwareResults：①hthenormalovaryeveryindexonlyexpressingranulosacellsofprimaryfolliclesandsecondaryfollicles，buthasnoexpressionintheprimitiveandmaturefolliclesTheratiosofhypo—expressio

6、nofWT1proteinandAMHbetweentheprimaryfolliclesandthesecondaryfolliclesweresignificantlydifferent(P005)TheratiosofexpressionofWT1proteinandAMHinovariangranulosacelltumourare811％and784％②Theratiosofhypo—expressionofWT1protei

7、nandAMHintherecrudescentpatientsandthepatientswithasciteswerelowerthanthatintheunrecrudescentpatientsandthepatientswithoutofascites，andthedifferencehasstatisticalsignificance(P／3／10HPFandthetumourswithconspicuousheteromo

8、rphismofcellswerelowerthanthatintumoursofwhichthehistotypeisn’tasystematic，inthecasesofcaryocinesis3／10HPFandthetumourswithoutofconspicuousheteromorphismofcells，andthedifferencehasstatisticalsignificance(P005)Theprolifer

9、ationindexofKi一67werealsoconcemedwiththetumourshistotype，thetumourcells’caryocinesisandheteromorphism(P005)③TheratiosofpositiveexpressionofWT1proteinandAMHinstage1werehigherthanthatinstageIIandstageIII(PO01)Theproliferat

10、ionindexofKi67wasalsoconcernedwiththeclinicalstage(P001)@Thetumourshistotype，thetumourcells’caryocinesisandheteromorphismwereconcernedwiththetumoursrecidivationandclinicalstageInthepatientsofstageI，theratiooftherecrudesc