微生物發(fā)酵產(chǎn)物LMC對卵巢癌細胞抑制作用的研究.pdf

1、天津大學碩士學位論文微生物發(fā)酵產(chǎn)物LMC對卵巢癌細胞抑制作用的研究姓名：王雪茵申請學位級別：碩士專業(yè)：生藥學指導教師：高文遠蔡兵20080101ABSTRACTThispaperfocusedonthestudyoftheanticanceractivityofLMCinvitroLMCisacompoundextractedfromthefermentationproductsofmicroorganismsAtfirst，wedet

2、ectedtheLMContheproliferationactivityofA2780bySRBassayTheICs0ValueofLMCforA2780was：13760385pMTheproliferationofA2780(oneoftheHumanOvarianCancerCellLines)wasinhibitedbyLMCinadosagedependentmannerandthisinhibitioneffectwas

3、increasedobviouslyathigherdosagesover1uM(including1uM)WedetectedtheLMCeffectonA2780celllinebyFCM，itwasfoundthatthecellpercentageofsub—G0／GIphasewasremainbasicallyunchangedandthecellpercentageofG0／G1phasewassignificantlyi

4、ncreasedThechangesofcellpercentagewerethetime—dependentmannerTheresultsindicatedthattheinhibitionofLMConcellproliferationwasnotcausedbyapoptosis，butshouldbearoleG0／GIphasearrestWealsotestedtheLMCeffectonA2780／cDDPcelllin

5、ebyFCMTheresultsshowedthatLMChadastronginhibitioneffectoncelllineA2780／cDDPandtheinhibitionmechanismwasalsotheG0／G1phasearrestMoreovertheinhibitionratesofLMCweresimilaronbothcell1inesA2780andA2780／cDDPWesternblottingassa

6、yresultsshowedthat。aftertreatmentwith1pMofLMC，P21andP27werefoundtObeincreasednoticeably，CyclinDwasdecreasedsignificantlyCyclinEalsodecreasdlightlyAlloftheabovechangesweretime—dependentIncaseofotherrelatedproteinssuchasP5