水產(chǎn)品中甲苯咪唑殘留量測定方法的研究.pdf

1、福建農(nóng)林大學(xué)碩士學(xué)位論文水產(chǎn)品中甲苯咪唑殘留量測定方法的研究姓名：曹愛英申請學(xué)位級別：碩士專業(yè)：水產(chǎn)品加工與貯藏指導(dǎo)教師：吳成業(yè)陳麗嬌20080401StudyonthedeterminationofmebendazoleresidueinaquaticproductAbstractMebendazole(MEB)，hardlysolubleinwaterisabroad—spectruminsecticidewiththenature

2、oflowtoxicitytomammalsAppliedbothinhumanandanimals，EMBcanusuallybeservedasakindofgastrointestinaldrugforanimalsandfishInaddition，itcarlalsobeusedtOkillthephylaAnnelidainfishAtpresentthemethodsusedfordetectingmebendazolei

3、ncludetitrationUVspectrophotometrycolorimetryphosphorescencespectrophotometryfluorescencespectrophotometryinfraredspectrophotometrythinlayerchromatographyandhighperformanceliquidchromatographyHowevermostofthesemethodsale

4、usedtodetectthecontentofmebendazolecontainedinmebendazoletrochesortheamountofmebendazoleintheblood，whilefewofthemareusedtodetecttheresidueofmebendazoleinaquaticproducts：。Andthesemethodshaveahighdetectionlimitwhichcanhard

5、lymeetthestandard一2099／kgstipulatedintheconfirmativelistestablishedbyJapanThispaperhassystematicallystudiedthedetectionmethodsofmebendazoleinaquaticproductswiththeaidofhighperformanceliquidchromatographyandthentheoptimum

6、detectionconditionWasobtainedaccordingtothecharacteristicofHPLCandthenatureofmebendazoleInthispapertheconditionofchromatographiccolumnmobilephase，ratioofmobilephase，longestabsorptionwavelengthandcolumntemperature，whichmi

7、ghthaveasignificanteffectontheresults，werechosenasvariablefactorstodeterminethechromatographicconditionofHPLCThroughtestofprecision，detectinglimitsamplestabilityandspikedstandardexperiment，wehavecheckedthemethodologyofth

8、emethodBymeansofexperiments，theoptimumchromatographyconditionchromatographycolumnusingantiphaseMightysilRPI8chromatographycolumn(250mmx46mm，5岬)；mobilephase，03％phosphoricacid—acetonitrilemixedsolution(6535，03％acidsolution

9、usingethylaminetoadjustPHto25)；mobilespeed，10m1／min；detectionwavelength，247nm；columntemperature，35“CTheresultsindicatedthatthemebendazolewithconcentrationofbetween011tg／mL一2499／mLhaveagoodlinealrelation(r=09988)Thedetect

10、ionlimitofthemethodis10pg／kgwhichislowerthan2099／kgstipulatedinthecortfirmativelistestablishedbyJapanUsingpreviousmethodtoperformexperimentsontherecoveryrateofcommonaquaticproducts，andtherecoveryrateisbetween8642％and9682

11、％，theaveragerelativestandarddevimionis158％ThispaperalsodiscussedthefactorsthataffectuncertaintyduringthemeasurementintheHPLC，thengiveananalysisandevaluationonit，SOastogainacompletelyrationeddescriptionofthemeasurementres