貽貝生物活性的研究.pdf

1、分類號題目浮≈爰。毛碩士學(xué)位論文英文并列題目：貽貝生物活性的研究密級StudiesOnBioactivitiesofMytUusEdudlsLinneas研究生：奎芏芏專業(yè)：盒晶登堂研究方向：丞簽煎盒疊堂一導(dǎo)師：工置墨塾亟指導(dǎo)小組成員學(xué)位授予日期：谷文英徐德平二00六年三月答辯委員會(huì)主席：張憨教攆江南大學(xué)地址：無錫市二00六年三月江南大學(xué)碩士學(xué)位論文Mytilusspprocessingwaste，whichisabundantinwa

2、tersolublecomponentsofmussel，isaliquidbyproductgeneratedintheindusuialsteamtreatmentofmusselFewpaperswerereportedabouttheresearchandexploitationofMytilusspprocessingwasteTheaimofthisthesisistheisolation，purificationofpol

3、ysaccharidesandtaurine，manufactureofnaturalsaucefromMytilusedudislinneasprocessingwaste巾BM)Thecrudepolysaccharide巾BMP)isolatedfromDBMexhibitedobviousbioactivitiesnleisolatingtechnologyconditionswereoptimizedbysinglefacto

4、rexperimentorthogonalexperimentandrespondsurfacemethodologyandtheresultswereshownasfollowingsDeproteination：DBMWaSdigestedbyO52％(v／w)BacillusSubtilisneutralproteasefor3hunderthetechnologyconditionofpH70，41℃，O74％(w／w)CaCl

5、2；thenSevagereagent，CHCl3：nBuOH(v／v)=5：1Wasaddedtemovetheresidualproteinfor20minandtheratioofsampletoSevageis4：l(v／v)TheamountofproteinremovedWas785％EtOHprecipitation：95％EtOHWasaddedtotheconcentratedsolutionuntiltheconce

6、ntrationofEtOHreached80％Dec010ration：theaqueoussolutionWasdec010redby1％(w／v)activecharcoalundertheconditionofpH30，50℃，20minDBⅣ呼dissolvedinwaterwasfractionedbyaionexchangechromatographyonacolumnofDEAEcelluloseandthenpurif

7、iedonacolumnofSepharoseCL一6BDBⅣⅢ11exhibitingsignificantimmunocompetencewasobtainedDBⅣ田11Wasidentifiedbygelpermeationchromatography(GPC)，hi曲pressureliquidchromatography(HPLC)，infraredspectroscopy(IR)，ultravioletscanningsp

8、ectroscopy(US)andgaschromatography(GC)TheresultsshowedthatDBMP1lWasahomogeneous130lysaecharide；itsweight—averagemolecularweight(M、Ⅳ)Wasabout524407Da：theIRspectrumshowedcharacteristicabsorptiousofcarbohydratepatternsbetwe

9、en500and4000cml，BconfigurationandQ—configurationwerealSepresentedbytheabsorptionat928cm“‘a(chǎn)nd858cml：itwascomposedofxylose，glucoseandgalactosewithaproportionof022：535：003andglucoseWasthepredominantpartDBMWasallidealmateria

10、ltoproducetaurineItWasextracteddwithion—exchangeresinand也enthesolutionwasconcentrated也eproductWascrystallizedoutwithethanolinlowtemperatureItsyieldCOuldreach052％ThesuitableextractiontechnologyoftaurineWaSstudiedand也eresu

11、ltWasthevolumeofsample5ml，pH45，elutingrate5ml／mintheamountoftaurineextractionwas9438％ThemanufactureofanamralsaucefromDBMwasreportedThenutritiousproducthaduniqueflavorandcontainedabundanttaurineandactivepolysaccharides，wh