窒息新生鼠腦核因子-κB的表達及地塞米松的影響.pdf

1、青島大學碩士學位論文窒息新生鼠腦核因子κB的表達及地塞米松的影響姓名：初建芳申請學位級別：碩士專業(yè)：兒科學指導教師：徐書珍20050524英文摘要TheexpressionofNFkuappaBandtheeffectofdexamethasoneinbrainofnewbornratswithasphyxiaAbstractiObjectiveTOobservetheactivationofnuclearfactorkappaB(NF

2、B)andtheeffectofdexamethasoneonNF一BactivityinbrainofnewbornratswithasphyxiaTheaimofthestudyistOexploretheeffectofNF一BsignalpathwayinneonatwithasphyxiaSOas協(xié)providetheexperimentalbaseforfindingclinicaltreatmentofasphyxiain

3、whichNFBistakenasthetargetMethodsnewbornrats(7daysold)wererandomlydividedintothreegroupscontrolgroup，asphyxiagroupandDXMpretreatmentgroupThemodelofasphyxiawasinducedinasphyxiagroupandDXMprelreatmentgroupImmunohistochemic

4、almethodwasusedtodetecttheactivityofNFBp65inthebrainofthenewbornrats，andhematoxylineosinstainedwasalsoperformtoobservethehistologicalevidenceofasphyxiainthebrainResultsTheactivityofNFBp65WaSstrongerintheaSphyxiagroupthan

5、thatinthecontrolgroup(pO05)，increasedtothehighestpeakat5d；AfterDXMpretreatmentNFBp65activitybecamelower(p005)；TheneurollshadhistologicalevidenceofapoptosisinasphyxiagroupHistologicalevidenceofapuptosiswasalsoobservedinDX

6、Mpmtreatmentgroup，butlessintenselyConclusionsTheresultssuggestthatNFBmayplayanimportantroleinbraininjurywithasphysia；DXMhavesonicprotectiveeffectsagainstthebraininjuryandthatprotectionseemedtoberelatedtoinlfibitingaugmen