熒光定量PCR檢測外周血中CK19、CK20 mRNA與乳腺癌微轉(zhuǎn)移的相關(guān)性研究.pdf

1、青島大學(xué)碩士學(xué)位論文熒光定量PCR檢測外周血中CK19、CK20mRNA與乳腺癌微轉(zhuǎn)移的相關(guān)性研究姓名：鄭妮申請學(xué)位級別：碩士專業(yè)：病原生物學(xué)指導(dǎo)教師：王斌20050528青島犬學(xué)碗上學(xué)位論文AssociationbetweenCKl9andCK20mRNAandthemierometastasisofbreastcaflcerbyfluorescentquantificationpolymerasechainreactionAbstr

2、actobjective：Toevaluatetheassociationbetweencytokeration19(CKl91mRNAandcytokeration20(CK20)mRNAwiththemicrometastasisinthecirculatingcellsofbreastcancerbyfluomscentquantificationpolymerasechainreaction(FQ—PCR)Methods：CKl

3、9andCK20mRNAintheofclinicalsamplesofl5healthwomen，20patientswithbenignbreastdiseaseand31patientswithbreastcancerweredetectedbyFQ—PCRGAPDHWasusedasinternalcontrolsThemeasurementdataWasanalyzedstatisticlybyQ—testandenumera

4、tiondatabychi—squeretestResults：TherewerenosignificantdifferenceinCKl9mRNAandCKl9／GAPDHbetweennormalcontrolsandbenignbreastdiseasegroup(pO05)CKl9mRNAandCKl9／GAPDHinbreastcancergroupwerehigherthanthoseintheothergroups(pO0

5、5)ThepercentofpositiveofCKl9mRNAandCKl9／GAPDHin31breastcantx：rpatientswere613％(19／31)Inthedetectionofcytokeration20(CK20)mRNA，therewerenosignificantdifferenceamongthreegroups(p005)，andthepercentofpositiveofCK20mRNAandCK2

6、0／GAPDHin3lbreastcancerpatientswere3％(1／31)Therewerenosignificantdifferencebetweenpre—operationand1weekafteroperationinCKl9mRNAandCK20mRNA(pO05)ThelaterWaslowerthantheformerConclusions：FQPCRisarapid，sensitiveandspecificm

7、ethodforquantitatingCKl9andCK20mRNAThedetectiveofCKl9mRNAgivesobjecfiveevidencetothemicrometastasisofbreastcancerPostgraduatestndent：ZhengNi(Pathogeuology)DirectedbyProfWangBinKeywords：Fluorescentquantificationpolymerase