散發(fā)性結(jié)腸癌3號染色體等位基因雜合缺失.pdf

1、復(fù)旦大學(xué)碩士學(xué)位論文散發(fā)性結(jié)腸癌3號染色體等位基因雜合缺失姓名：凌云申請學(xué)位級別：碩士專業(yè)：外科學(xué)指導(dǎo)教師：彭志海2001.4.1LossofHeterozygosityonChromosome3inSporadicColorectalCarcinomaABSTRACTobjeetive：Tostudythelossofheterozygosity(LOH)onchromosome3insporadiccolorectalcarcino

2、ma(SCRC)，exploringthepossiblecolorectalcancerrelatedgenesorlociMethods：LOHat13microsatellitelocispanningcllromosome3weredetectedin83casesofSCRCSCorrelationbetweentheLOHresultsandclinicpathologydatawasanalyzedResults：Micr

3、osatelliteinstability(MSI)wasdetectedin9casesofSCRCs，andintheremained74MSInegativecases，29(39％)casesweredetectedtohaveatleasttwolOCiwithLOHonchmmosome3，27(37％)caseswerefoundtohaveatleastonelOCUSwitllLOHon3p，and39(53％)Cas

4、eshadatleastonelOCUS誚t11LoHon3q0fthe13loeiexamined，D3S1300(3p142、Wasidentified髂thelocuswithmostfrequentLoH3qLoHWasfoundtooccurindistalcolorectalcancelsmoreoftenthanthoseproximaltothespleenflexTumorswith3pand／orD3S1300LOH

5、haveatendencyoflocalinvasionandoccurredmoreofteninpatientsolderthan50yearsConclusion：LOHoncllromosome3，dispersedwithhighfrequencyatseveralloci，wascorrelatedwithclinicpathologyofSCRC，indicatingtheexistenceofSCRCrelatedgen

6、esonitThediscoveryofhighLOHfrequencyatD3SI300suggestedthatFHITgenelocatedinthisregionmightactasacandidatetumorsuppressorgeneinthedevelopmentofcolorectalcarcinomaKeyWords：CarcinomaColorectal；Chromosome；LossofHeterozygosit