活化的肝星狀細(xì)胞在肝癌微環(huán)境中的調(diào)節(jié)作用.pdf

1、廈門大學(xué)碩士學(xué)位論文活化的肝星狀細(xì)胞在肝癌微環(huán)境中的調(diào)節(jié)作用姓名：張磊申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：外科學(xué)指導(dǎo)教師：王效民201105AbstractBackgroundandaimsHepatocellularcarcinoma(HCC)isacommonworldwidemalignancyHoweverComprehensivetreatmentisfollowedbypoorprognosisThepurposeofimmunothe

2、rapyistobreaklocalRLrnorimmunetoleranceorimmunesuppression，improvethebody’Simmunefunction，whichmakestheimmunoproph)rlaxisofHCCAccumulatingrepotssuggestedthathepaticstellatecells(HSCs)exhibitedtheabilityinimmunosuppressio

3、nandimmunoregulation，andshouldberesponsiblefortheoccurrenceanddevelopmentofhepatocellularcarcinoma(HCC)MethodsInthisstudyHSCswereisolatedfromBalb／emiceToestablishastableprimaryhepatocellularcarcinoma(HCC)modelmice，andest

4、ablishabasisfordepthlaboratoryresearchinhepatocellularcancerWeimmunohistochemicallyexaminedfortheirinfluencesonH22proliferation，angiogenesis，Lymphangiogenesis，Tcells，expressionofB7H1，tumorassociatedmacrophagecells(TAMcel

5、ls)intumormicroenvironmentFurthermore，weexaminedTlymphocytesubsetsinsplenocyteweredetectedbyFCMResuRsWedemonstratedCotransplantationofHSCspromotedHCCgrowthandprogressionbyenhancingtumorangiogenesis，Lymphangiogenesisandtu

6、morcellproliferationHSCsinhibitedthelymphocyteinfiltrationintumorsandthespleensofmicebearingtumors，inducedapoptosisofTcells，andenhancedtheexpressionofB7HlandTAMcellsConclusionOurresultsdemonstratedthatHSCspromoteHCCprogr

7、essionbecauseofitsimmuneregulatoryactivitypartiallyOurdatasupplynewinformationtosupportanintegralroleforHSCsinpromotingHCCprogressionandHSCsmayserveasatherapeutictargetforHCCKeywordsHepaticstellatecells，Hepatocellularcar